| Title |
In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, November 2010
|
| DOI | 10.1007/s11626-010-9366-3 |
| Pubmed ID | |
| Authors |
S. Richelle Monaghan, Rebecca L. Rumney, Nguyen T. K. Vo, Niels C. Bols, Lucy E. J. Lee |
| Abstract |
Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish skin and brain respectively, ZEB2J from zebrafish embryos, FHMT-W1 from fathead minnow testis, and Sf9 from ovaries of a fall armyworm moth. All cultures were maintained at 27°C. Infection was judged to have taken place by the appearance of sporonts and/or spores in cells and occurred in all cell lines. Spores were also isolated from ZEB2J cultures and used to successfully infect new cultures of ZEB2J, RK-13 and Sf9. These results suggest that cells of a wide range of vertebrates support A. algerae growth in vitro and fish cells can produce spores infectious to cells of mammals, fish, and insects. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| Unknown | 35 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 28% |
| Student > Master | 7 | 19% |
| Researcher | 5 | 14% |
| Student > Bachelor | 2 | 6% |
| Professor | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 9 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 42% |
| Veterinary Science and Veterinary Medicine | 4 | 11% |
| Medicine and Dentistry | 4 | 11% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Unknown | 10 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 July 2015.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#156
of 790 outputs
Outputs of similar age
#53,956
of 180,109 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#5
of 7 outputs
Altmetric has tracked 22,790,780 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 790 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,109 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.