| Title |
Characterization of Bacillus spp. strains for use as probiotic additives in pig feed
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, November 2013
|
| DOI | 10.1007/s00253-013-5343-6 |
| Pubmed ID | |
| Authors |
Nadja Larsen, Line Thorsen, Elmer Nayra Kpikpi, Birgitte Stuer-Lauridsen, Mette Dines Cantor, Bea Nielsen, Elke Brockmann, Patrick M. F. Derkx, Lene Jespersen |
| Abstract |
Bacillus spp. are commonly used as probiotic species in the feed industry, however, their benefits need to be confirmed. This study describes a high throughput screening combined with the detailed characterization of endospore-forming bacteria with the aim to identify new Bacillus spp. strains for use as probiotic additives in pig feed. A total of 245 bacterial isolates derived from African fermented food, feces and soil were identified by 16S rRNA gene sequencing and screened for antimicrobial activity and growth in the presence of antibiotics, bile salts and at pH 4.0. Thirty-three Bacillus spp. isolates with the best characteristics were identified by gyrB and rpoB gene sequencing as B. amyloliquefaciens subsp. plantarum, B. amyloliquefaciens subsp. amyloliquefaciens, B. subtilis subsp. subtilis, B. licheniformis, B. mojavensis, B. pumilus and B. megaterium. These isolates were further investigated for their activity against the pathogenic bacteria, antibiotic susceptibility, sporulation rates, biofilm formation and production of glycosyl hydrolytic enzymes. Additionally, ten selected isolates were assessed for heat resistance of spores and the effect on porcine epithelial cells IPEC-J2. Isolates of B. amyloliquefaciens, B. subtilis and B. mojavensis, showed the best overall characteristics and, therefore, potential for usage as probiotic additives in feed. A large number of taxonomically diverse strains made it possible to reveal species and subspecies-specific trends, contributing to our understanding of the probiotic potential of Bacillus species. |
Mendeley readers
The data shown below were compiled from readership statistics for 163 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 1% |
| Unknown | 161 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 27 | 17% |
| Student > Master | 24 | 15% |
| Student > Ph. D. Student | 23 | 14% |
| Student > Bachelor | 16 | 10% |
| Student > Doctoral Student | 7 | 4% |
| Other | 20 | 12% |
| Unknown | 46 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 56 | 34% |
| Biochemistry, Genetics and Molecular Biology | 21 | 13% |
| Immunology and Microbiology | 9 | 6% |
| Veterinary Science and Veterinary Medicine | 7 | 4% |
| Medicine and Dentistry | 6 | 4% |
| Other | 14 | 9% |
| Unknown | 50 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 February 2023.
All research outputs
#3,545,582
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#535
of 8,034 outputs
Outputs of similar age
#33,095
of 220,958 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#7
of 95 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 95 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.