| Title |
Steady state growth space study of Lactococcus lactis in D-stat cultures
|
|---|---|
| Published in |
Antonie van Leeuwenhoek, July 2009
|
| DOI | 10.1007/s10482-009-9363-2 |
| Pubmed ID | |
| Authors |
Petri-Jaan Lahtvee, Kaspar Valgepea, Ranno Nahku, Kristo Abner, Kaarel Adamberg, Raivo Vilu |
| Abstract |
Growth space of Lactococcus lactis subsp. lactis IL1403 was studied at constant growth rate using D-stat cultivation technique. Starting from steady state conditions in a chemostat culture (mu = 0.2 h(-1)), the pH and/or temperature were continuously changed in the range of 5.4-6.4 and 26-34 degrees C, respectively, followed by the return to the initial environmental conditions. Based on substrate consumption and product formation yields and expression changes of 1,920 genes, it was shown that changes of physiological state were not dependent on the direction of movement (from pH 6.3 to 5.4 or from 5.4 to 6.3), showing that quasi steady state values in D-stat corresponded to the steady state values in chemostats. Relative standard deviation of growth characteristics in triplicate D-stat experiments was below 10%. Continuing the experiment and reestablishing initial growth conditions revealed in average 7% difference (hysteresis) in growth characteristics when comparing chemostat steady state cultures prior and after the change of environmental conditions. Similarly, shifts were also seen at gene expression levels. The large amount of quantitatively reliable data obtained in this study provided a new insight into dynamic properties of bacterial physiology, and can be used for describing the growth space of microorganisms by modeling cell metabolism. |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 2 | 6% |
| Australia | 1 | 3% |
| Netherlands | 1 | 3% |
| Denmark | 1 | 3% |
| Estonia | 1 | 3% |
| Unknown | 29 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 34% |
| Student > Ph. D. Student | 6 | 17% |
| Student > Master | 6 | 17% |
| Other | 3 | 9% |
| Professor | 2 | 6% |
| Other | 5 | 14% |
| Unknown | 1 | 3% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 60% |
| Biochemistry, Genetics and Molecular Biology | 6 | 17% |
| Engineering | 3 | 9% |
| Mathematics | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 2 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2014.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from Antonie van Leeuwenhoek
#534
of 2,024 outputs
Outputs of similar age
#37,036
of 110,039 outputs
Outputs of similar age from Antonie van Leeuwenhoek
#5
of 7 outputs
Altmetric has tracked 22,790,780 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,024 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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