Title |
Detection and identification of Bacteriovorax stolpii UKi2 sphingophosphonolipid molecular species
|
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Published in |
Journal of the American Society for Mass Spectrometry, March 2007
|
DOI | 10.1016/j.jasms.2006.10.014 |
Pubmed ID | |
Authors |
Koka Jayasimhulu, Shannon M. Hunt, Edna S. Kaneshiro, Yoko Watanabe, José-Luis Giner |
Abstract |
Bacteriovorax stolpii is a predator of larger gram-negative bacteria and lives as a parasite in the intraperiplasmic space of the host cell. This bacterium is unusual among prokaryotes in that sphingolipids comprise a large proportion of its lipids. We here report the presence of 18 molecular species of B. stolpii UKi2 sphingophosphonolipids (SPNLs). (31)P NMR spectroscopy and analysis of P(i) released by a differential hydrolysis protocol confirmed the phosphonyl nature of these lipids. The SPNLs were dominated by those with 1-hydroxy-2-aminoethane phosphonate (hydroxy-aminoethylphosphonate) polar head groups; aminoethylphosphonate was also detected in minor SPNL components. The long-chain bases (LCBs) were dominated by C(17) iso-branched phytosphingosine; C(17) iso-branched dihydrosphingosine was also present in some SPNLs. The N-linked fatty acids were predominantly iso-branched and most contained an alpha-hydroxy group (C(15) alpha-hydroxy fatty acid was the major fatty acid). Minor molecular species containing nonhydroxy fatty acids were also detected. The definitive iso-structures of the predominant fatty acids and LCBs present in the B. stolpii SPNLs were established using (13)C and (3)H nuclear magnetic resonance spectroscopy; less than 20% were unbranched. Detection and analyses of intact compounds by MS-MS were performed by a hybrid quadrupole time-of-flight (Q-TOF-II) MS equipped with an electrospray ionization source. Analyses of peracetylated derivatives verified the structural assignments of these lipids. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 30% |
Researcher | 6 | 30% |
Professor > Associate Professor | 3 | 15% |
Student > Master | 1 | 5% |
Unknown | 4 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 20% |
Chemistry | 4 | 20% |
Immunology and Microbiology | 3 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Environmental Science | 1 | 5% |
Other | 2 | 10% |
Unknown | 4 | 20% |