Report for: High Throughput Screening

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Chapter title	Automated patch clamping using the QPatch.
Chapter number	10
Book title	High Throughput Screening
Published in	Methods in molecular biology, June 2009
DOI	10.1007/978-1-60327-258-2_10
Pubmed ID	19551364
Book ISBNs	978-1-60327-257-5, 978-1-60327-258-2
Authors	Kenneth A. Jones, Nicoletta Garbati, Hong Zhang, Charles H. Large
Abstract	Whole-cell voltage clamp electrophysiology using glass patch pipettes (1) is regarded as the gold standard for measurement of compound activity on ion channels. Despite the high quality of the data generated by this method, in its traditional format, patch clamping has limited use in drug screening due to very low throughput. Over the years, developments in microfabrication have driven the development of planar, multi-aperture technologies that are suitable for parallel, automated patch recording techniques. Here we present detailed methods for two common applications of the planar patch technology using one of the commercially available instruments. The results demonstrate (a) the high quality of whole-cell recordings obtainable from cell lines expressing human Nav1.2 or hERG ion channels, (b) the advantages of the methodology for increasing throughput, and (c) examples of how these assays support ion channel drug discovery.

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Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
United States	2	7%
Mexico	1	4%
Unknown	24	89%

Demographic breakdown

Readers by professional status	Count	As %
Researcher	10	37%
Student > Ph. D. Student	4	15%
Student > Master	3	11%
Other	2	7%
Lecturer	2	7%
Other	3	11%
Unknown	3	11%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	8	30%
Pharmacology, Toxicology and Pharmaceutical Science	3	11%
Biochemistry, Genetics and Molecular Biology	3	11%
Physics and Astronomy	3	11%
Engineering	3	11%
Other	4	15%
Unknown	3	11%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2012.

All research outputs

#7,454,951

of 22,790,780 outputs

Outputs from Methods in molecular biology

#2,318

of 13,110 outputs

Outputs of similar age

#37,510

of 111,347 outputs

Outputs of similar age from Methods in molecular biology

of 20 outputs

Altmetric has tracked 22,790,780 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.

So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 76% of its peers.

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High Throughput Screening

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Geographical breakdown

Demographic breakdown

Attention Score in Context