Title |
Flavonoid Metabolites in the Hemolymph of European Pine Sawfly (Neodiprion sertifer) Larvae
|
---|---|
Published in |
Journal of Chemical Ecology, April 2012
|
DOI | 10.1007/s10886-012-0113-y |
Pubmed ID | |
Authors |
Matti Vihakas, Petri Tähtinen, Vladimir Ossipov, Juha-Pekka Salminen |
Abstract |
Flavonoids in the hemolymph of European pine sawfly (Neodiprion sertifer) larvae that were feeding on Pinus sylvestris needles were identified. HPLC-ESI-MS analysis revealed that the main components in the hemolymph were flavonol di- and triglucosides and a catechin monoglucoside. These compounds were isolated from the larval hemolymph and their structures were established by HPLC-MS, GC-MS, and NMR spectroscopy. The isolated flavonoids were identified as (+)-catechin 7-O-β-glucoside, isorhamnetin 3,7,4'-tri-O-β-glucoside, kaempferol 3,7,4'-tri-O-β-glucoside, and quercetin 3,7,4'-tri-O-β-glucoside. The combined concentration of these four compounds in the hemolymph was 3.7 mg/ml. None of these compounds was present in the needles of P. sylvestris. Therefore, we propose that the flavonoid glucosides were produced by the larvae from flavonoid monoglucosides and (+)-catechin obtained from the pine needles. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 29% |
Student > Master | 4 | 17% |
Student > Ph. D. Student | 3 | 13% |
Student > Bachelor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 3 | 13% |
Unknown | 3 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 38% |
Chemistry | 9 | 38% |
Materials Science | 1 | 4% |
Unknown | 5 | 21% |