| Title |
A novel 4-hydroxycoumarin biosynthetic pathway
|
|---|---|
| Published in |
Plant Molecular Biology, September 2009
|
| DOI | 10.1007/s11103-009-9548-0 |
| Pubmed ID | |
| Authors |
Benye Liu, Torben Raeth, Till Beuerle, Ludger Beerhues |
| Abstract |
Coumarin forms in melilotoside (trans-ortho-coumaric acid glucoside)-containing plant species upon cell damage. In moldy melilotoside-containing plant material, trans-ortho-coumaric acid is converted by fungi to 4-hydroxycoumarin, two molecules of which spontaneously combine with formaldehyde to give dicoumarol. Dicoumarol causes internal bleeding in livestock and is the forerunner of the warfarin group of medicinal anticoagulants. Here, we report 4-hydroxycoumarin formation by biphenyl synthase (BIS). Two new BIS cDNAs were isolated from elicitor-treated Sorbus aucuparia cell cultures. The encoded isoenzymes preferred ortho-hydroxybenzoyl (salicoyl)-CoA as a starter substrate and catalyzed a single decarboxylative condensation with malonyl-CoA to give 4-hydroxycoumarin. When elicitor-treated S. aucuparia cell cultures were fed with the N-acetylcysteamine thioester of salicylic acid, 4-hydroxycoumarin accumulated in the culture medium. Incubation of the BIS isoenzymes with benzoyl-CoA and malonyl-CoA resulted in the formation of 3,5-dihydroxybiphenyl which is the precursor of the phytoalexins of the Maloideae. |
Mendeley readers
The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 2% |
| Unknown | 49 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 32% |
| Researcher | 4 | 8% |
| Student > Doctoral Student | 4 | 8% |
| Student > Master | 4 | 8% |
| Professor | 3 | 6% |
| Other | 8 | 16% |
| Unknown | 11 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 13 | 26% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 14% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Agricultural and Biological Sciences | 6 | 12% |
| Chemical Engineering | 3 | 6% |
| Other | 3 | 6% |
| Unknown | 11 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2021.
All research outputs
#8,759,452
of 25,837,817 outputs
Outputs from Plant Molecular Biology
#1,046
of 2,919 outputs
Outputs of similar age
#38,718
of 108,562 outputs
Outputs of similar age from Plant Molecular Biology
#2
of 10 outputs
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