| Title |
Purification and characterization of UDP-glucose: anthocyanin 3′,5′-O-glucosyltransferase from Clitoria ternatea
|
|---|---|
| Published in |
Planta, August 2007
|
| DOI | 10.1007/s00425-007-0584-1 |
| Pubmed ID | |
| Authors |
Koichiro Kogawa, Naoki Kato, Kohei Kazuma, Naonobu Noda, Masahiko Suzuki |
| Abstract |
A UDP-glucose: anthocyanin 3',5'-O-glucosyltransferase (UA3'5'GT) (EC 2.4.1.-) was purified from the petals of Clitoria ternatea L. (Phaseoleae), which accumulate polyacylated anthocyanins named ternatins. In the biosynthesis of ternatins, delphinidin 3-O-(6''-O-malonyl)-beta-glucoside (1) is first converted to delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-glucoside (2). Then 2 is converted to ternatin C5 (3), which is delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-glucoside. UA3'5'GT is responsible for these two steps by transferring two glucosyl groups in a stepwise manner. Its substrate specificity revealed the regioselectivity to the anthocyanin's 3'- or 5'-OH groups. Its kinetic properties showed comparable k (cat) values for 1 and 2, suggesting the subequality of these anthocyanins as substrates. However, the apparent Km value for 1 (3.89 x 10(-5) M), which is lower than that for 2 (1.38 x 10(-4) M), renders the k(cat)/Km value for 1 smaller, making 1 catalytically more efficient than 2. Although the apparent Km value for UDP-glucose (6.18 x 10(-3) M) with saturated 2 is larger than that for UDP-glucose (1.49 x 10(-3) M) with saturated 1, the k(cat) values are almost the same, suggesting the UDP-glucose binding inhibition by 2 as a product. UA3'5'GT turns the product 2 into a substrate possibly by reversing the B-ring of 2 along the C2-C1' single bond axis so that the 5'-OH group of 2 can point toward the catalytic center. |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 2% |
| India | 1 | 2% |
| Australia | 1 | 2% |
| Unknown | 50 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 21% |
| Student > Bachelor | 8 | 15% |
| Lecturer | 4 | 8% |
| Professor > Associate Professor | 4 | 8% |
| Professor | 3 | 6% |
| Other | 11 | 21% |
| Unknown | 12 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 32% |
| Biochemistry, Genetics and Molecular Biology | 9 | 17% |
| Engineering | 7 | 13% |
| Chemistry | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 14 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2022.
All research outputs
#4,888,383
of 23,515,383 outputs
Outputs from Planta
#221
of 2,785 outputs
Outputs of similar age
#13,325
of 67,919 outputs
Outputs of similar age from Planta
#5
of 24 outputs
Altmetric has tracked 23,515,383 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,785 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 67,919 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.