| Title |
Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase
|
|---|---|
| Published in |
Molecular Genetics and Genomics, August 1999
|
| DOI | 10.1007/s004380051040 |
| Pubmed ID | |
| Authors |
R. A. Geremia, M. Roux, D. U. Ferreiro, R. Dauphin-Dubois, A. C. Lellouch, L. Ielpi |
| Abstract |
Biosynthesis of repeat-unit polysaccharides and N-linked glycans proceeds by sequential transfer of sugars from the appropriate sugar donor to an activated lipid carrier. The transfer of each sugar is catalysed by a specific glycosyltransferase. The molecular basis of the specificity of sugar addition is not yet well understood, mainly because of the difficulty of isolating these proteins. In this study, the aceA gene product expressed by Acetobacter xylinum, which is involved in the biosynthesis of the exopolysaccharide acetan, was overproduced in Escherichia coli and its function was characterised. The aceA ORF was subcloned into the expression vector pET29 in frame with the S.tag epitope. The recombinant protein was identified, and culture conditions were optimised for production of the soluble protein. The results of test reactions showed that AceA is able to transfer one alpha-mannose residue from GDP-mannose to cellobiose-P-P-lipid to produce alpha-mannose-cellobiose-P-P-lipid. AceA was not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 6% |
| Canada | 1 | 6% |
| Unknown | 15 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 29% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Doctoral Student | 2 | 12% |
| Student > Master | 2 | 12% |
| Student > Bachelor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 3 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 41% |
| Biochemistry, Genetics and Molecular Biology | 4 | 24% |
| Philosophy | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Chemistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 3 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 March 2013.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Molecular Genetics and Genomics
#920
of 3,319 outputs
Outputs of similar age
#11,275
of 34,663 outputs
Outputs of similar age from Molecular Genetics and Genomics
#4
of 19 outputs
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