Title |
Characterization of the psychrotolerant acetogen strain SyrA5 and the emended description of the species Acetobacterium carbinolicum
|
---|---|
Published in |
Antonie van Leeuwenhoek, December 2005
|
DOI | 10.1007/s10482-005-9009-y |
Pubmed ID | |
Authors |
Maiken Paarup, Michael W. Friedrich, Brian J. Tindall, Kai Finster |
Abstract |
A psychrotolerant, obligate anaerobic, acetogenic bacterium designated strain SyrA5 was isolated from black anoxic sediment of a brackish fjord. Cells were Gram-positive, non-sporeforming rods. The isolate utilized H(2)/CO(2), CO, fructose, glucose, ethanol, ethylene glycol, glycerol, pyruvate, lactate, betaine and the methyl-groups of several methoxylated benzoic derivatives such as syringate, trimethoxybenzoate and vallinate. The optimum temperature for growth was 29 degrees C, whilst slow growth occurred at 2 degrees C. The strain grew optimally with NaCl concentrations below 2.7% (w/v), but growth occurred up to 4.3% (w/v) NaCl. Growth was observed in the range from pH 5.9 to 8.5, optimum at pH 8. The G+C content was 44.1 mol%. Based upon 16S rRNA gene sequence analysis and DNA-DNA reassociation studies, the organism was classified in the genus Acetobacterium. Strain SyrA5 shared a 16S rRNA sequence similarity with A. carbinolicum of 100%, a fthfs gene (which codes for the N5,N10 tetrahydrofolate synthetase) sequence identity of 98.5-98.7% (amino acid sequence similarities were 99.4-100%) and a RNA-DNA hybridization homology of 64-68%. Despite a number of phenotypic differences between strain SyrA5 and A. carbinolicum we propose including strain SyrA5 as a subspecies of A. carbinolicum for which we propose the name Acetobacterium carbinolicum subspecies kysingense. The type strain is SyrA5 (=DSM 16427(T), ATCC BAA-990). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 25% |
Student > Ph. D. Student | 8 | 22% |
Student > Master | 5 | 14% |
Student > Doctoral Student | 3 | 8% |
Student > Bachelor | 2 | 6% |
Other | 5 | 14% |
Unknown | 4 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 17 | 47% |
Environmental Science | 6 | 17% |
Engineering | 2 | 6% |
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 4 | 11% |
Unknown | 5 | 14% |