Title |
Applications of Loop-Mediated Isothermal DNA Amplification
|
---|---|
Published in |
Applied Biochemistry and Biotechnology, September 2010
|
DOI | 10.1007/s12010-010-9088-8 |
Pubmed ID | |
Authors |
Shijun Fu, Guanggang Qu, Shijin Guo, Lin Ma, Na Zhang, Songlin Zhang, Sanyang Gao, Zhiqiang Shen |
Abstract |
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification. |
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