| Title |
Electric ultrafreezer (− 150 °C) as an alternative for zebrafish sperm cryopreservation and storage
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, April 2018
|
| DOI | 10.1007/s10695-018-0500-6 |
| Pubmed ID | |
| Authors |
Patrícia Diogo, Gil Martins, Isa Quinzico, Rita Nogueira, Paulo J. Gavaia, Elsa Cabrita |
| Abstract |
Zebrafish sperm cryopreservation is a fundamental methodology to manage and back-up valuable genetic resources like transgenic and mutant strains. Cryopreservation usually requires liquid nitrogen for storage, which is expensive and hazardous. Our objective was to evaluate if electric ultrafreezers (- 150 °C) are a viable alternative for zebrafish sperm storage. Zebrafish sperm was cryopreserved in the same conditions (- 20 °C/min), stored either in liquid nitrogen or in an ultrafreezer, and thawed after 1 week, 1 month, and 3 months. Sperm motility, membrane integrity, and fertilization ability were assessed. There were no significant differences in motility and hatching rate throughout storage time. Additionally, we aimed at understanding if cryopreservation directly in an ultrafreezer (- 66 °C/min) could improve post-thaw sperm quality. Freezing at - 20 °C/min was performed as before, and compared to samples cryopreserved with a fast cooling rate by placing directly in an ultrafreezer (- 66 °C/min). Sperm quality was assessed according to motility, viability, DNA fragmentation, and apoptosis (annexin V). The - 66 °C/min cooling rate showed significantly higher membrane and DNA integrity, and lower number of cells in late apoptosis in comparison to the other treatments. This study showed that zebrafish sperm cryopreservation and storage in an ultrafreezer system is possible and a fast cooling rate directly in ultrafreezer improves post-thaw sperm quality. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Fiji | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 15% |
| Student > Doctoral Student | 7 | 13% |
| Student > Ph. D. Student | 6 | 12% |
| Researcher | 5 | 10% |
| Student > Bachelor | 4 | 8% |
| Other | 7 | 13% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 35% |
| Veterinary Science and Veterinary Medicine | 4 | 8% |
| Biochemistry, Genetics and Molecular Biology | 4 | 8% |
| Medicine and Dentistry | 3 | 6% |
| Environmental Science | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 17 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2018.
All research outputs
#18,603,172
of 23,043,346 outputs
Outputs from Fish Physiology and Biochemistry
#414
of 867 outputs
Outputs of similar age
#254,519
of 327,991 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#11
of 40 outputs
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