| Title |
Differential miRNA expression and their target genes between NGX6-positive and negative colon cancer cells
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, September 2010
|
| DOI | 10.1007/s11010-010-0582-7 |
| Pubmed ID | |
| Authors |
Xiao-Yan Wang, Ming-Hua Wu, Fen Liu, Yu Li, Nan Li, Gui-Yuan Li, Shou-Rong Shen |
| Abstract |
Nasopharyngeal carcinoma-associated gene 6 (NGX6) was shown to be a novel putative tumor suppressor gene in colon cancer. The purpose of this study is to investigate its role in regulation of miRNA expression for in the hopes of translating this data into a novel strategy in control of colon cancer. In this study colon cancer HT-29 cells were stably transfected with NGX6 or vector-only plasmid and then subjected to miRNA array analysis, and Q-RT-PCR was then used to verify miRNA array data. Then bioinformatic analyses using Sanger, Target Scan, and MicroRNA software were performed to obtain data on the target genes of each miRNA and define their function. Our results showed that 14 miRNAs were found to be differentially expressed in NGX6-transfected cells compared to the control cells. In particular, miR-126, miR-142-3p, miR-155, miR-552, and miR-630 were all upregulated, whereas miR-146a, miR-152, miR-205, miR-365, miR-449, miR-518c, miR-584, miR-615, and miR-622 were downregulated after NGX6 transfection. Q-RT-PCR confirmed all of these miRNAs, and invalidated miR-552 and miR-630. Furthermore, bioinformatic analyses of these 12 miRNAs, among these miRNAs, target genes of miR-615 are unclear, another 11 miRNAs produced a total of 254 potential target genes and further study showed that these genes together formed a regulatory network that contributes to apoptosis, mobility/migration, hydrolysis activity, and molecular signaling through targeting JNK and Notch pathways. Taken together, these results have suggested that NGX6 plays an important role in regulation of apoptosis, mobility/migration, and hydrolase as well as activity of JNK and Notch pathways through NGX6-mediated miRNA expression. Further investigation will reveal the function of these differentially expressed miRNAs and verify expression of the miRNA-targeted genes for development of novel strategies for better control of colon cancer. |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 2% |
| Norway | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 39 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 36% |
| Researcher | 9 | 21% |
| Student > Master | 4 | 10% |
| Professor | 3 | 7% |
| Student > Doctoral Student | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 4 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 31% |
| Medicine and Dentistry | 10 | 24% |
| Biochemistry, Genetics and Molecular Biology | 9 | 21% |
| Computer Science | 2 | 5% |
| Nursing and Health Professions | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 5 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2023.
All research outputs
#3,353,784
of 23,230,825 outputs
Outputs from Molecular and Cellular Biochemistry
#112
of 2,337 outputs
Outputs of similar age
#13,431
of 97,985 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#2
of 13 outputs
Altmetric has tracked 23,230,825 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,337 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.