| Title |
MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation
|
|---|---|
| Published in |
International Journal of Legal Medicine, February 2010
|
| DOI | 10.1007/s00414-009-0402-3 |
| Pubmed ID | |
| Authors |
Dmitry Zubakov, Anton W. M. Boersma, Ying Choi, Patricia F. van Kuijk, Erik A. C. Wiemer, Manfred Kayser |
| Abstract |
MicroRNAs (miRNAs) are non-protein coding molecules with important regulatory functions; many have tissue-specific expression patterns. Their very small size in principle makes them less prone to degradation processes, unlike messenger RNAs (mRNAs), which were previously proposed as molecular tools for forensic body fluid identification. To identify suitable miRNA markers for forensic body fluid identification, we first screened total RNA samples derived from saliva, semen, vaginal secretion, and venous and menstrual blood for the expression of 718 human miRNAs using a microarray platform. All body fluids could be easily distinguished from each other on the basis of complete array-based miRNA expression profiles. Results from quantitative reverse transcription PCR (RT-PCR; TaqMan) assays for microarray candidate markers confirmed strong over-expression in the targeting body fluid of several miRNAs for venous blood and several others for semen. However, no candidate markers from array experiments for other body fluids such as saliva, vaginal secretion, or menstrual blood could be confirmed by RT-PCR. Time-wise degradation of venous blood and semen stains for at least 1 year under lab conditions did not significantly affect the detection sensitivity of the identified miRNA markers. The detection limit of the TaqMan assays tested for selected venous blood and semen miRNA markers required only subpicogram amounts of total RNA per single RT-PCR test, which is considerably less than usually needed for reliable mRNA RT-PCR detection. We therefore propose the application of several stable miRNA markers for the forensic identification of blood stains and several others for semen stain identification, using commercially available TaqMan assays. Additional work remains necessary in search for suitable miRNA markers for other forensically relevant body fluids. |
Mendeley readers
The data shown below were compiled from readership statistics for 301 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | <1% |
| Malaysia | 1 | <1% |
| France | 1 | <1% |
| China | 1 | <1% |
| Spain | 1 | <1% |
| Unknown | 296 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 51 | 17% |
| Student > Master | 50 | 17% |
| Student > Ph. D. Student | 49 | 16% |
| Researcher | 37 | 12% |
| Student > Postgraduate | 13 | 4% |
| Other | 31 | 10% |
| Unknown | 70 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 70 | 23% |
| Agricultural and Biological Sciences | 65 | 22% |
| Medicine and Dentistry | 30 | 10% |
| Chemistry | 18 | 6% |
| Engineering | 5 | 2% |
| Other | 27 | 9% |
| Unknown | 86 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2023.
All research outputs
#3,194,156
of 22,792,160 outputs
Outputs from International Journal of Legal Medicine
#130
of 2,061 outputs
Outputs of similar age
#17,709
of 166,330 outputs
Outputs of similar age from International Journal of Legal Medicine
#1
of 7 outputs
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