| Title |
Tissue protein imaging at 1 μm laser spot diameter for high spatial resolution and high imaging speed using transmission geometry MALDI TOF MS
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2015
|
| DOI | 10.1007/s00216-015-8532-6 |
| Pubmed ID | |
| Authors |
Andre Zavalin, Junhai Yang, Kevin Hayden, Marvin Vestal, Richard M. Caprioli |
| Abstract |
We have achieved protein imaging mass spectrometry capabilities at sub-cellular spatial resolution and at high acquisition speed by integrating a transmission geometry ion source with time of flight mass spectrometry. The transmission geometry principle allowed us to achieve a 1-μm laser spot diameter on target. A minimal raster step size of the instrument was 2.5 μm. Use of 2,5-dihydroxyacetophenone robotically sprayed on top of a tissue sample as a matrix together with additional sample preparation steps resulted in single pixel mass spectra from mouse cerebellum tissue sections having more than 20 peaks in a range 3-22 kDa. Mass spectrometry images were acquired in a standard step raster microprobe mode at 5 pixels/s and in a continuous raster mode at 40 pixels/s. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 122 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 2 | 2% |
| Unknown | 120 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 37 | 30% |
| Researcher | 21 | 17% |
| Student > Master | 11 | 9% |
| Student > Doctoral Student | 10 | 8% |
| Student > Bachelor | 9 | 7% |
| Other | 9 | 7% |
| Unknown | 25 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 43 | 35% |
| Biochemistry, Genetics and Molecular Biology | 16 | 13% |
| Agricultural and Biological Sciences | 10 | 8% |
| Medicine and Dentistry | 6 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 4% |
| Other | 14 | 11% |
| Unknown | 28 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 June 2023.
All research outputs
#5,187,899
of 25,394,764 outputs
Outputs from Analytical & Bioanalytical Chemistry
#775
of 9,624 outputs
Outputs of similar age
#70,360
of 367,600 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#9
of 129 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,624 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 367,600 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 129 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.