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N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential

Overview of attention for article published in Scientific Reports, January 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

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1 X user
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2 patents

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Title
N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential
Published in
Scientific Reports, January 2015
DOI 10.1038/srep08003
Pubmed ID
Authors

Krystal Teasley Hamorsky, J. Calvin Kouokam, Jessica M. Jurkiewicz, Bailey Nelson, Lauren J. Moore, Adam S. Husk, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba

Abstract

Plant-based transient overexpression systems enable rapid and scalable production of subunit vaccines. Previously, we have shown that cholera toxin B subunit (CTB), an oral cholera vaccine antigen, is N-glycosylated upon expression in transgenic Nicotiana benthamiana. Here, we found that overexpression of aglycosylated CTB by agroinfiltration of a tobamoviral vector causes massive tissue necrosis and poor accumulation unless retained in the endoplasmic reticulum (ER). However, the re-introduction of N-glycosylation to its original or an alternative site significantly relieved the necrosis and provided a high CTB yield without ER retention. Quantitative gene expression analysis of PDI, BiP, bZIP60, SKP1, 26Sα proteasome and PR1a, and the detection of ubiquitinated CTB polypeptides revealed that N-glycosylation significantly relieved ER stress and hypersensitive response, and facilitated the folding/assembly of CTB. The glycosylated CTB (gCTB) was characterized for potential vaccine use. Glycan profiling revealed that gCTB contained approximately 38% plant-specific glycans. gCTB retained nanomolar affinity to GM1-ganglioside with only marginal reduction of physicochemical stability and induced an anti-cholera holotoxin antibody response comparable to native CTB in a mouse oral immunization study. These findings demonstrated gCTB's potential as an oral immunogen and point to a potential role of N-glycosylation in increasing recombinant protein yields in plants.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 1%
Germany 1 1%
Unknown 68 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 23%
Researcher 15 21%
Student > Master 10 14%
Student > Bachelor 4 6%
Student > Postgraduate 4 6%
Other 9 13%
Unknown 12 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 30%
Biochemistry, Genetics and Molecular Biology 21 30%
Chemical Engineering 5 7%
Medicine and Dentistry 4 6%
Pharmacology, Toxicology and Pharmaceutical Science 3 4%
Other 5 7%
Unknown 11 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2019.
All research outputs
#4,584,268
of 23,073,835 outputs
Outputs from Scientific Reports
#35,453
of 124,696 outputs
Outputs of similar age
#66,173
of 352,950 outputs
Outputs of similar age from Scientific Reports
#290
of 1,120 outputs
Altmetric has tracked 23,073,835 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 124,696 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,950 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 1,120 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.