Title |
MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-κB Pathway
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Published in |
Cell Host & Microbe (Science Direct), February 2015
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DOI | 10.1016/j.chom.2015.01.007 |
Pubmed ID | |
Authors |
Manish Kumar, Sanjaya Kumar Sahu, Ranjeet Kumar, Arijita Subuddhi, Ranjan Kumar Maji, Kuladip Jana, Pushpa Gupta, Johanna Raffetseder, Maria Lerm, Zhumur Ghosh, Geert van Loo, Rudi Beyaert, Umesh D. Gupta, Manikuntala Kundu, Joyoti Basu |
Abstract |
The outcome of the interaction between Mycobacterium tuberculosis (Mtb) and a macrophage depends on the interplay between host defense and bacterial immune subversion mechanisms. MicroRNAs critically regulate several host defense mechanisms, but their role in the Mtb-macrophage interplay remains unclear. MicroRNA profiling of Mtb-infected macrophages revealed the downregulation of miR-let-7f in a manner dependent on the Mtb secreted effector ESAT-6. We establish that let-7f targets A20, a feedback inhibitor of the NF-κB pathway. Expression of let-7f decreases and A20 increases with progression of Mtb infection in mice. Mtb survival is attenuated in A20-deficient macrophages, and the production of TNF, IL-1β, and nitrite, which are mediators of immunity to Mtb, is correspondingly increased. Further, let-7f overexpression diminishes Mtb survival and augments the production of cytokines including TNF and IL-1β. These results uncover a role for let-7f and its target A20 in regulating immune responses to Mtb and controlling bacterial burden. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | <1% |
Canada | 1 | <1% |
Brazil | 1 | <1% |
Unknown | 149 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 32 | 21% |
Student > Ph. D. Student | 27 | 18% |
Student > Master | 17 | 11% |
Student > Bachelor | 15 | 10% |
Student > Postgraduate | 9 | 6% |
Other | 22 | 14% |
Unknown | 30 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 34 | 22% |
Immunology and Microbiology | 32 | 21% |
Biochemistry, Genetics and Molecular Biology | 27 | 18% |
Medicine and Dentistry | 10 | 7% |
Veterinary Science and Veterinary Medicine | 4 | 3% |
Other | 16 | 11% |
Unknown | 29 | 19% |