| Title |
First Report of a New Isolate of Metarhizium rileyi from Maize Fields of Quivicán, Cuba
|
|---|---|
| Published in |
Indian Journal of Microbiology, March 2018
|
| DOI | 10.1007/s12088-018-0721-5 |
| Pubmed ID | |
| Authors |
Sandra Pérez Álvarez, Amaury Méndez Guerrero, Bernardo Nayar Débora Duarte, Marco Antonio Magallanes Tapia, Jesús Alicia Chávez Medina, Yoannis Domínguez Rodríguez |
| Abstract |
Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S. frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification. The amplified products of 1335 bp were purified and sequenced at CINVESTAV-IPN in both the directions using the above primers. A consensus sequence was obtained by alignment of the forward and reverse sequences for this region and deposited in GenBank (MG637450). The fungus produced slightly cottony colony of pale green color and dispersed conidia and septal mycelium were observed under the optical microscope. A BLAST search of the sequence in GenBank revealed a 99% of identity with several strains of N. rileyi (e.g., AF368501.1, AB268359.1 and EU553337.1) and M. rileyi (e.g., KY436756.1). This is the first report of M. rileyi isolate from maize fields of Quivicán in Cuba and this is important for biodiversity studies and is another possibility for Integrated Pest Management. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 5 | 19% |
| Student > Master | 4 | 15% |
| Researcher | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Lecturer | 1 | 4% |
| Other | 5 | 19% |
| Unknown | 7 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 56% |
| Biochemistry, Genetics and Molecular Biology | 1 | 4% |
| Unspecified | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 30% |
Attention Score in Context
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All research outputs
#18,603,172
of 23,043,346 outputs
Outputs from Indian Journal of Microbiology
#244
of 384 outputs
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#258,261
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Outputs of similar age from Indian Journal of Microbiology
#8
of 17 outputs
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