Title |
Neurological heterotopic ossification following spinal cord injury is triggered by macrophage‐mediated inflammation in muscle
|
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Published in |
The Journal of Pathology, March 2015
|
DOI | 10.1002/path.4519 |
Pubmed ID | |
Authors |
François Genêt, Irina Kulina, Cedryck Vaquette, Frédéric Torossian, Susan Millard, Allison R Pettit, Natalie A Sims, Adrienne Anginot, Bernadette Guerton, Ingrid G Winkler, Valérie Barbier, Jean-Jacques Lataillade, Marie-Caroline Le Bousse-Kerdilès, Dietmar W Hutmacher, Jean-Pierre Levesque |
Abstract |
Neurological heterotopic ossification (NHO) is the abnormal formation of bone in soft tissues as a consequence of spinal cord or traumatic brain injury. NHO causes pain, ankyloses, vascular and nerve compression and delays rehabilitation in this high morbidity patient group. The pathological mechanisms leading to NHO remain unknown and consequently there are no therapeutic options to prevent or reduce NHO. Genetically modified mouse models of rare genetic forms of heterotopic ossification (HO) exist but their relevance to NHO is questionable. Consequently we developed the first model of spinal cord injury (SCI)-induced NHO in genetically unmodified mice. Formation of NHO, measured by micro-computerized tomography, required the combination of both SCI and localized muscular inflammation. Our NHO model faithfully reproduced many clinical features of NHO in SCI patients and both human and mouse NHO tissues contained macrophages. Muscle-derived mesenchymal progenitors underwent osteoblast differentiation in vitro in response to serum from NHO mice without additional exogenous osteogenic stimuli. Substance P was identified as a candidate NHO systemic neuropeptide as it was significantly elevated in the serum of NHO patients. However antagonism of substance P receptor in our NHO model only modestly reduced the volume of NHO. In contrast, ablation of phagocytic macrophages with clodronate-loaded liposomes reduced the size of NHO by 90%, supporting the conclusion that NHO is highly dependent on inflammation and phagocytic macrophages in soft tissues. Overall, we have developed the first clinically relevant model of NHO and demonstrated that a combined insult of neurological injury and soft tissue inflammation drives NHO pathophysiology. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Netherlands | 3 | 43% |
Australia | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 57% |
Scientists | 2 | 29% |
Practitioners (doctors, other healthcare professionals) | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Unknown | 89 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 15 | 17% |
Student > Bachelor | 14 | 16% |
Researcher | 10 | 11% |
Student > Master | 7 | 8% |
Student > Doctoral Student | 7 | 8% |
Other | 18 | 20% |
Unknown | 19 | 21% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 31 | 34% |
Agricultural and Biological Sciences | 7 | 8% |
Neuroscience | 7 | 8% |
Immunology and Microbiology | 5 | 6% |
Nursing and Health Professions | 4 | 4% |
Other | 8 | 9% |
Unknown | 28 | 31% |