| Title |
The effect of resistant starch (RS) on the bovine rumen microflora and isolation of RS-degrading bacteria
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2018
|
| DOI | 10.1007/s00253-018-8971-z |
| Pubmed ID | |
| Authors |
Dong-Hyun Jung, Dong-Ho Seo, Ga-Young Kim, Young-Do Nam, Eun-Ji Song, Shawn Yoon, Cheon-Seok Park |
| Abstract |
Resistant starch (RS) in the diet reaches the large intestine without degradation, where it is decomposed by the commensal microbiota. The fermentation of RS produces secondary metabolites including short-chain fatty acids (SCFAs), which have been linked to a variety of physiological and health effects. Therefore, the availability of RS as a prebiotic is a current issue. The objectives of this study were (1) to use metagenomics to observe microbial flora changes in Bos taurus coreanae rumen fluid in the presence of RS and (2) to isolate RS-degrading microorganisms. The major microbial genus in a general rumen fluid was Succiniclasticum sp., whereas Streptococcus sp. immediately predominated after the addition of RS into the culture medium and was then drastically replaced by Lactobacillus sp. The presence of Bifidobacterium sp. was also observed continuously. Several microorganisms with high RS granule-degrading activity were identified and isolated, including B. choerinum FMB-1 and B. pseudolongum FMB-2. B. choerinum FMB-1 showed the highest RS-hydrolyzing activity and degraded almost 60% of all substrates tested. Coculture experiments demonstrated that Lactobacillus brevis ATCC 14869, which was isolated from human feces, could grow using reducing sugars generated from RS by B. choerinum FMB-1. These results suggest that Bifidobacterium spp., especially B. choerinum FMB-1, are the putative primary degrader of RS in rumen microbial flora and could be further studied as probiotic candidates. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 11% |
| United States | 1 | 11% |
| United Kingdom | 1 | 11% |
| Unknown | 6 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 67% |
| Scientists | 3 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 17% |
| Student > Ph. D. Student | 8 | 14% |
| Other | 7 | 12% |
| Lecturer | 6 | 10% |
| Student > Master | 5 | 9% |
| Other | 8 | 14% |
| Unknown | 14 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 38% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Immunology and Microbiology | 5 | 9% |
| Medicine and Dentistry | 3 | 5% |
| Chemistry | 2 | 3% |
| Other | 7 | 12% |
| Unknown | 14 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2018.
All research outputs
#2,814,293
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#398
of 8,034 outputs
Outputs of similar age
#57,825
of 331,716 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#8
of 141 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,716 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 141 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.