| Title |
Structural predication and antigenic analysis of Toxoplasma gondii ROP20
|
|---|---|
| Published in |
Acta Parasitologica, April 2018
|
| DOI | 10.1515/ap-2018-0028 |
| Pubmed ID | |
| Authors |
Jian Zhou, Wanchun Wang, Pengxia Song, Lin Wang, Yali Han, Jingjing Guo, Zhen Hao, Xi Zhu, Qiong Zhou, Xiadong Du, Gang Lu, Shenyi He, Yingquan Luo |
| Abstract |
Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii. In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online procedure and Visual Molecular Dynamics (VMD) software. The structure analysis fully indicated that ROP20 protein is an important member of the ROP family. Furthermore, We used DNASTAR software and Epitope Database online service to analyze liner-B cell epitopes and T-cell epitopes of ROP20 protein. All the analysis results of ROP20 protein can provide positive information on treatment and vaccine for toxoplasmosis. Moreover, ROP20 gene was obtained from PCR, and a recombinant eukaryotic expression vector (pEGFP-C1-ROP20) was constructed in the following study. After restriction enzyme digestion, the constructed plasmid was transfected into HEK 293-T cells. The RT-PCR result indicated that the recombinant plasmid could transcribe successfully in HEK 293-T cell. The results of western blotting indicated the expressed proteins can be recognized by anti-STAg mouse sera. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 22% |
| Student > Ph. D. Student | 1 | 11% |
| Unspecified | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Student > Master | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 22% |
| Unspecified | 1 | 11% |
| Veterinary Science and Veterinary Medicine | 1 | 11% |
| Agricultural and Biological Sciences | 1 | 11% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2018.
All research outputs
#22,767,715
of 25,382,440 outputs
Outputs from Acta Parasitologica
#481
of 735 outputs
Outputs of similar age
#301,940
of 342,076 outputs
Outputs of similar age from Acta Parasitologica
#11
of 17 outputs
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