Title |
Tightening up the structure, lighting up the pathway: application of molecular constraints and light to manipulate protein folding, self-assembly and function
|
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Published in |
Science China Chemistry, October 2014
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DOI | 10.1007/s11426-014-5225-5 |
Pubmed ID | |
Authors |
Beatrice N. Markiewicz, Robert M. Culik, Feng Gai |
Abstract |
Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins. Recently, other types of molecular constraints, especially photoresponsive linkers and functional groups, have also found increased use in a wide variety of applications. Herein, we provide a concise review of using various forms of molecular strategies to constrain proteins, thereby stabilizing their native states, gaining insight into their folding mechanisms, and/or providing a handle to trigger a conformational process of interest with light. The applications discussed here cover a wide range of topics, ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function. |
X Demographics
Geographical breakdown
Country | Count | As % |
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India | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 26% |
Student > Ph. D. Student | 5 | 26% |
Professor | 2 | 11% |
Student > Master | 2 | 11% |
Professor > Associate Professor | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 16% |
Readers by discipline | Count | As % |
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Chemistry | 10 | 53% |
Agricultural and Biological Sciences | 3 | 16% |
Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Medicine and Dentistry | 1 | 5% |
Unknown | 3 | 16% |