| Title |
Multiplet-filtered and gradient-selected zero-quantum TROSY experiments for 13C1H3 methyl groups in proteins
|
|---|---|
| Published in |
Journal of Biomolecular NMR, September 2011
|
| DOI | 10.1007/s10858-011-9533-1 |
| Pubmed ID | |
| Authors |
Michelle L. Gill, Arthur G. Palmer |
| Abstract |
Multiplet-filtered and gradient-selected heteronuclear zero-quantum coherence (gsHZQC) TROSY experiments are described for measuring (1)H-(13)C correlations for (13)CH(3) methyl groups in proteins. These experiments provide improved suppression of undesirable, broad outer components of the heteronuclear zero-quantum multiplet in medium-sized proteins, or in flexible sites of larger proteins, compared to previously described HZQC sequences (Tugarinov et al. in J Am Chem Soc 126:4921-4925, 2004; Ollerenshaw et al. in J Biomol NMR 33:25-41, 2005). Hahn-echo versions of the gsHZQC experiment also are described for measuring zero- and double-quantum transverse relaxation rate constants for identification of chemical exchange broadening. Application of the proposed pulse sequences to Escherichia coli ribonuclease HI, with a molecular mass of 18 kD, indicates that improved multiplet suppression is obtained without substantial loss of sensitivity. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 4% |
| United States | 1 | 4% |
| Unknown | 24 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 35% |
| Student > Ph. D. Student | 5 | 19% |
| Professor | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Professor > Associate Professor | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 35% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Chemistry | 6 | 23% |
| Unknown | 4 | 15% |