| Title |
Efficient Biotransformation of Phytosterols to Dehydroepiandrosterone by Mycobacterium sp.
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, April 2018
|
| DOI | 10.1007/s12010-018-2739-x |
| Pubmed ID | |
| Authors |
Pei Zhou, Ya-kun Fang, Hao-ke Yao, Hua Li, Gang Wang, Yu-peng Liu |
| Abstract |
In this study, a method for the efficient production of dehydroepiandrosterone (DHEA) from phytosterols in a vegetable oil/aqueous two-phase system by Mycobacterium sp. was developed. After the 3-hydroxyl group of phytosterols was protected, they could be converted into DHEA with high yield and productivity by Mycobacterium sp. NRRL B-3683. In a shake flask biotransformation, 15.05 g l-1 of DHEA and a DHEA yield of 85.39% (mol mol-1) were attained after 7 days with an initial substrate concentration of 25 g l-1. When biotransformation was carried out in a 30-l stirred bioreactor with 25 g l-1 substrate, the DHEA concentration and yield was 16.33 g l-1 and 92.65% (mol mol-1) after 7 days, respectively. The results of this study suggest that inexpensive phytosterols could be utilized for the efficient production of DHEA. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 35% |
| Student > Doctoral Student | 2 | 12% |
| Professor > Associate Professor | 2 | 12% |
| Student > Bachelor | 2 | 12% |
| Other | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 24% |
| Chemistry | 3 | 18% |
| Chemical Engineering | 2 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2018.
All research outputs
#18,603,172
of 23,043,346 outputs
Outputs from Applied Biochemistry and Biotechnology
#1,797
of 2,532 outputs
Outputs of similar age
#254,212
of 327,682 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#10
of 23 outputs
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So far Altmetric has tracked 2,532 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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