| Title |
Genotyping and virulence analysis of Toxoplasma gondii isolates from a dead human fetus and dead pigs in Jiangsu province, Eastern China
|
|---|---|
| Published in |
Acta Parasitologica, April 2018
|
| DOI | 10.1515/ap-2018-0046 |
| Pubmed ID | |
| Authors |
Zhaofeng Hou, Yonghua Zhou, Dandan Liu, Shijie Su, Zhenxing Zhao, Jinjun Xu, Jianping Tao |
| Abstract |
Toxoplasma gondii is an obligate intracellular parasite with worldwide distribution. Virulence of T. gondii is a multigenic trait. Genetic and virulence data for T. gondii isolates from humans and animals in China have been reported. However, almost all biological materials used for genotyping of T. gondii from humans and pigs were DNA samples prepared from tissues, and T. gondii strains used for virulence analysis were isolated mainly from cats. In this study, one isolate from a dead human fetus was identified as type I (ToxoDB #10) while the two isolates from dead pigs were type Chinese I (ToxoDB #9) with PCR-restriction fragment length polymorphism using 10 markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Three isolates were comfirmed as virulent strains in mice. By cloning and sequences analysis, all isolates contained a Pvu II restriction site (572-577 bp) in the KHB fragment and five tandem repeats in the 5' UTR region of SAG1, which were associated with T. gondii virulence. The type Chinese I isolates contained two deletions of 15 and 3 bp at positions 635 to 649 and 658 to 660 in the GRA6, which were correlated with genotype, but not with virulence. To our knowledge, this is the first report on the systematic analysis of murine virulence of type Chinese I strain from pigs, and the associations of sequences of the KHB fragment and SAG1 with virulence of type Chinese I strain. The Chinese I genotype was more closely related to type II strains. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| South Africa | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Master | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Librarian | 1 | 8% |
| Researcher | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 17% |
| Environmental Science | 1 | 8% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Agricultural and Biological Sciences | 1 | 8% |
| Nursing and Health Professions | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 4 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2018.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Acta Parasitologica
#304
of 735 outputs
Outputs of similar age
#251,264
of 342,076 outputs
Outputs of similar age from Acta Parasitologica
#5
of 17 outputs
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So far Altmetric has tracked 735 research outputs from this source. They receive a mean Attention Score of 2.0. This one has gotten more attention than average, scoring higher than 51% of its peers.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.