Title |
Fluorometric RdRp assay with self-priming RNA
|
---|---|
Published in |
Virus Genes, March 2015
|
DOI | 10.1007/s11262-015-1187-8 |
Pubmed ID | |
Authors |
Fatih Kocabas, Raife D. Turan, Galip S. Aslan |
Abstract |
There is an outmost need for the identification of specific antiviral compounds. Current antivirals lack specificity, making them susceptible to off-target effects, and highlighting importance of development of assays to discover antivirals targeting viral specific proteins. Previous studies for identification of inhibitors of RNA-dependent RNA polymerase (RdRp) mostly relied on radioactive methods. This study describes a fluorometric approach to assess in vitro activity of viral RdRp for drug screening. Using readily available DNA- and RNA-specific fluorophores, we determined an optimum fluorometric approach that could be used in antiviral discovery specifically for RNA viruses by targeting RdRp. Here, we show that double-stranded RNA could be successfully distinguished from single-stranded RNA. In addition, we provide a strategy based on self-priming RNA to assess RdRp activity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 45 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 20% |
Student > Ph. D. Student | 8 | 18% |
Other | 4 | 9% |
Student > Master | 4 | 9% |
Student > Bachelor | 3 | 7% |
Other | 6 | 13% |
Unknown | 11 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 24% |
Agricultural and Biological Sciences | 10 | 22% |
Immunology and Microbiology | 2 | 4% |
Medicine and Dentistry | 2 | 4% |
Chemical Engineering | 1 | 2% |
Other | 2 | 4% |
Unknown | 17 | 38% |