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Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae

Overview of attention for article published in Applied and Environmental Microbiology, March 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Good Attention Score compared to outputs of the same age and source (69th percentile)

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2 X users
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1 patent

Citations

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44 Dimensions

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108 Mendeley
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1 CiteULike
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Title
Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae
Published in
Applied and Environmental Microbiology, March 2015
DOI 10.1128/aem.04144-14
Pubmed ID
Authors

Timothy C. R. Brennan, Thomas C. Williams, Benjamin L. Schulz, Robin W. Palfreyman, Jens O. Krömer, Lars K. Nielsen

Abstract

Monoterpenes are liquid hydrocarbons with applications ranging from flavor and fragrance to replacement jet fuel. Their toxicity, however, presents a major challenge for microbial synthesis. Here we evolved limonene tolerant yeast and sequenced six strains across the 200 generation evolutionary time course. Mutations were found in the tricalbin proteins Tcb2p and Tcb3p. Genomic reconstruction in the parent strain showed that truncation of a single protein (tTcb3p(1-989)), but not its complete deletion, was sufficient to recover the evolved phenotype improving limonene fitness by nine-fold. tTcb3p(1-989) increased tolerance towards two other monoterpenes (β-pinene and myrcene) by 11- and 8-fold, respectively, and tolerance towards the bio-jet fuel blend AMJ-700t (10% cymene, 50% limonene, 40% farnesene) by 4-fold. tTcb3p(1-989) is the first example of successful engineering of phase tolerance and creates opportunities for the production of the highly toxic C10 alkenes in yeast.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 108 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 22%
Student > Bachelor 15 14%
Researcher 13 12%
Student > Master 12 11%
Other 5 5%
Other 16 15%
Unknown 23 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 27%
Biochemistry, Genetics and Molecular Biology 27 25%
Engineering 11 10%
Chemical Engineering 5 5%
Chemistry 3 3%
Other 6 6%
Unknown 27 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 December 2021.
All research outputs
#7,205,554
of 25,374,917 outputs
Outputs from Applied and Environmental Microbiology
#7,340
of 19,160 outputs
Outputs of similar age
#77,610
of 273,814 outputs
Outputs of similar age from Applied and Environmental Microbiology
#47
of 160 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 19,160 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,814 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 160 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.