| Title |
MicroRNA-26b inhibits metastasis of osteosarcoma via targeting CTGF and Smad1
|
|---|---|
| Published in |
Tumor Biology, March 2015
|
| DOI | 10.1007/s13277-015-3305-6 |
| Pubmed ID | |
| Authors |
Guoqing Duan, Chunfeng Ren, Yuanmin Zhang, Shiqing Feng |
| Abstract |
Downregulation of miR-26b has been found in various cancers, but it has never been investigated in osteosarcoma. In this study, we demonstrated downregulation of miR-26b in osteosarcoma tissues, negatively correlated with the expression of connective tissue growth factor (CTGF) and Smad1. Luciferase reporter assay confirmed the interaction of miR-26b with the 3' untranslated regions (UTRs) of CTGF and Smad1. Transfection of miR-26b in osteosarcoma cells suppressed the expression of CTGF and Smad1, suggesting CTGF and Smad1 as direct targets of miR-26b. Overexpression of miR-26b inhibited the migration of osteosarcoma cells, which was reversed by overexpression of CTGF or Smad1. Knockdown of CTGF by small interfering RNA (siRNA) interference blocked the activation of Smad1, ERK1/2, and MMP2, which was opposite to the overexpression of CTGF. Differently, Smad1 did not significantly affect CTGF level, but mediated ERK1/2 phosphorylation and MMP2 activation. Furthermore, miR-26b inhibited lung metastasis of osteosarcoma in vivo. Our data indicated that downregulation of miR-26b in osteosarcoma elevated the levels of CTGF and Smad1, facilitating osteosarcoma metastasis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 33% |
| Student > Ph. D. Student | 2 | 11% |
| Student > Postgraduate | 2 | 11% |
| Researcher | 2 | 11% |
| Student > Doctoral Student | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 5 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 28% |
| Agricultural and Biological Sciences | 3 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Sports and Recreations | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 6 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2015.
All research outputs
#20,264,045
of 22,794,367 outputs
Outputs from Tumor Biology
#1,834
of 2,622 outputs
Outputs of similar age
#218,698
of 259,041 outputs
Outputs of similar age from Tumor Biology
#96
of 164 outputs
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