| Title |
A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples
|
|---|---|
| Published in |
Medical Microbiology and Immunology, November 2007
|
| DOI | 10.1007/s00430-007-0063-0 |
| Pubmed ID | |
| Authors |
Lutz Eric Lehmann, Klaus-Peter Hunfeld, Thomas Emrich, Gerd Haberhausen, Heimo Wissing, Andreas Hoeft, Frank Stüber |
| Abstract |
Early detection of bloodstream infections (BSI) is crucial in the clinical setting. Blood culture remains the gold standard for diagnosing BSI. Molecular diagnostic tools can contribute to a more rapid diagnosis in septic patients. Here, a multiplex real-time PCR-based assay for rapid detection of 25 clinically important pathogens directly from whole blood in <6 h is presented. Minimal analytical sensitivity was determined by hit rate analysis from 20 independent experiments. At a concentration of 3 CFU/ml a hit rate of 50% was obtained for E. aerogenes and 100% for S. marcescens, E. coli, P. mirabilis, P. aeruginosa, and A. fumigatus. The hit rate for C. glabrata was 75% at 30 CFU/ml. Comparing PCR identification results with conventional microbiology for 1,548 clinical isolates yielded an overall specificity of 98.8%. The analytical specificity in 102 healthy blood donors was 100%. Although further evaluation is warranted, our assay holds promise for more rapid pathogen identification in clinical sepsis. |
Mendeley readers
The data shown below were compiled from readership statistics for 216 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | <1% |
| United States | 2 | <1% |
| Spain | 2 | <1% |
| United Kingdom | 1 | <1% |
| Belgium | 1 | <1% |
| Unknown | 208 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 44 | 20% |
| Student > Master | 36 | 17% |
| Student > Ph. D. Student | 32 | 15% |
| Student > Bachelor | 17 | 8% |
| Student > Doctoral Student | 16 | 7% |
| Other | 41 | 19% |
| Unknown | 30 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 57 | 26% |
| Medicine and Dentistry | 43 | 20% |
| Biochemistry, Genetics and Molecular Biology | 25 | 12% |
| Engineering | 17 | 8% |
| Immunology and Microbiology | 13 | 6% |
| Other | 24 | 11% |
| Unknown | 37 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 December 2022.
All research outputs
#3,998,480
of 23,815,455 outputs
Outputs from Medical Microbiology and Immunology
#60
of 627 outputs
Outputs of similar age
#10,009
of 65,515 outputs
Outputs of similar age from Medical Microbiology and Immunology
#2
of 4 outputs
Altmetric has tracked 23,815,455 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 627 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 65,515 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.