| Title |
Behavior tests and immunohistochemical retinal response analyses in RCS rats with subretinal implantation of Okayama-University-type retinal prosthesis
|
|---|---|
| Published in |
Journal of Artificial Organs, March 2013
|
| DOI | 10.1007/s10047-013-0697-1 |
| Pubmed ID | |
| Authors |
Alamusi, Toshihiko Matsuo, Osamu Hosoya, Kimiko M. Tsutsui, Tetsuya Uchida |
| Abstract |
We have developed a photoelectric dye-coupled polyethylene film as a prototype of retinal prosthesis, which we named Okayama University-type retinal prosthesis. The purposes of this study are to conduct behavior tests to assess vision in Royal College of Surgeons (RCS) rats that underwent subretinal implantation of the dye-coupled film and to reveal retinal response to the dye-coupled film by immunohistochemistry. Polyethylene films were made of polyethylene powder at refined purity, and photoelectric dyes were coupled to the film surface at higher density compared with the prototype. Either dye-coupled film or dye-uncoupled plain film used as a control was implanted subretinally from a scleral incision in both eyes of an RCS rat at 6 weeks of the age. Behavior tests 2, 4, 6, and 8 weeks after implantation were conducted by observing head turning or body turning in the direction consistent with clockwise or counterclockwise rotation of a black-and-white-striped drum around a transparent cage housed with the rat. After the behavior tests at 8 weeks, rats' eyes were enucleated to confirm subretinal implantation of the films and processed for immunohistochemistry. In the behavior tests, the number of head turnings consistent with the direction of the drum rotation was significantly larger in RCS rats with dye-coupled- compared with plain-film implantation [P < 0.05, repeated-measure analysis of variance (ANOVA), n = 7]. The number of apoptotic neurons was significantly smaller in eyes with dye-coupled- compared with plain-film implantation (P < 0.05, Mann-Whitney U test, n = 6). In conclusion, subretinal implantation of photoelectric dye-coupled films restored vision in RCS rats and prevented the remaining retinal neurons from apoptosis. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 11% |
| United States | 1 | 11% |
| Switzerland | 1 | 11% |
| Unknown | 6 | 67% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Student > Master | 2 | 22% |
| Researcher | 2 | 22% |
| Professor > Associate Professor | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 4 | 44% |
| Medicine and Dentistry | 2 | 22% |
| Neuroscience | 2 | 22% |
| Unknown | 1 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2023.
All research outputs
#1,747,400
of 23,972,269 outputs
Outputs from Journal of Artificial Organs
#6
of 261 outputs
Outputs of similar age
#14,153
of 200,471 outputs
Outputs of similar age from Journal of Artificial Organs
#1
of 2 outputs
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So far Altmetric has tracked 261 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 98% of its peers.
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