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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography
|
|---|---|
| Published in |
Nature Medicine, July 2010
|
| DOI | 10.1038/nm.2185 |
| Pubmed ID | |
| Authors |
Tarik F Massoud, Ramasamy Paulmurugan, Sanjiv S Gambhir |
| Abstract |
Improved techniques to noninvasively image protein-protein interactions (PPIs) are essential. We molecularly engineered a positron emission tomography (PET)-based split reporter (herpes simplex virus type 1 thymidine kinase), cleaved between Thr265 and Ala266, and used this in a protein-fragment complementation assay (PCA) to quantify PPIs in mammalian cells and to microPET image them in living mice. An introduced point mutation (V119C) markedly enhanced thymidine kinase complementation in PCAs, on the basis of rapamycin modulation of FKBP12-rapamycin-binding domain (FRB) and FKBP12 (FK506 binding protein), the interaction of hypoxia-inducible factor-1alpha with the von Hippel-Lindau tumor suppressor, and in an estrogen receptor intramolecular protein folding assay. Applications of this unique split thymidine kinase are potentially far reaching, including, for example, considerably more accurate monitoring of immune and stem cell therapies, allowing for fully quantitative and tomographic PET localization of PPIs in preclinical small- and large-animal models of disease. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Spain | 1 | 1% |
| Japan | 1 | 1% |
| Unknown | 85 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 25% |
| Student > Ph. D. Student | 18 | 20% |
| Student > Master | 12 | 13% |
| Professor > Associate Professor | 6 | 7% |
| Professor | 6 | 7% |
| Other | 12 | 13% |
| Unknown | 13 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 25% |
| Biochemistry, Genetics and Molecular Biology | 19 | 21% |
| Medicine and Dentistry | 11 | 12% |
| Chemistry | 9 | 10% |
| Engineering | 6 | 7% |
| Other | 9 | 10% |
| Unknown | 13 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2021.
All research outputs
#3,515,553
of 26,017,215 outputs
Outputs from Nature Medicine
#4,922
of 9,421 outputs
Outputs of similar age
#13,806
of 109,466 outputs
Outputs of similar age from Nature Medicine
#26
of 78 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,421 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 105.2. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,466 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 78 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.