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Mendeley readers
Attention Score in Context
| Title |
Microfluidic device for a rapid immobilization of Zebrafish larvae in environmental scanning electron microscopy
|
|---|---|
| Published in |
Cytometry Part A, December 2014
|
| DOI | 10.1002/cyto.a.22603 |
| Pubmed ID | |
| Authors |
Jin Akagi, Feng Zhu, Joanna Skommer, Chris J. Hall, Philip S. Crosier, Michal Cialkowski, Donald Wlodkowic |
| Abstract |
Small vertebrate model organisms have recently gained popularity as attractive experimental models that enhance our understanding of human tissue and organ development. Despite a large body of evidence using optical spectroscopy for the characterization of small model organism on chip-based devices, no attempts have been so far made to interface microfabricated technologies with environmental scanning electron microscopy (ESEM). Conventional scanning electron microscopy requires high vacuum environments and biological samples must be, therefore, submitted to many preparative procedures to dehydrate, fix, and subsequently stain the sample with gold-palladium deposition. This process is inherently low-throughput and can introduce many analytical artifacts. This work describes a proof-of-concept microfluidic chip-based system for immobilizing zebrafish larvae for ESEM imaging that is performed in a gaseous atmosphere, under low vacuum mode and without any need for sample staining protocols. The microfabricated technology provides a user-friendly and simple interface to perform ESEM imaging on zebrafish larvae. Presented lab-on-a-chip device was fabricated using a high-speed infrared laser micromachining in a biocompatible poly(methyl methacrylate) thermoplastic. It consisted of a reservoir with multiple semispherical microwells designed to hold the yolk of dechorionated zebrafish larvae. Immobilization of the larvae was achieved by a gentle suction generated during blotting of the medium. Trapping region allowed for multiple specimens to be conveniently positioned on the chip-based device within few minutes for ESEM imaging. © 2014 International Society for Advancement of Cytometry. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 29% |
| Researcher | 5 | 18% |
| Student > Master | 3 | 11% |
| Professor | 2 | 7% |
| Student > Bachelor | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 4 | 14% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Agricultural and Biological Sciences | 3 | 11% |
| Chemical Engineering | 2 | 7% |
| Medicine and Dentistry | 2 | 7% |
| Other | 4 | 14% |
| Unknown | 10 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2015.
All research outputs
#20,000,155
of 24,577,646 outputs
Outputs from Cytometry Part A
#1,172
of 1,391 outputs
Outputs of similar age
#272,779
of 370,217 outputs
Outputs of similar age from Cytometry Part A
#16
of 22 outputs
Altmetric has tracked 24,577,646 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,391 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 370,217 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.