| Title |
Cloning of Toluene 4-Monooxygenase Genes and Application of Two-Phase System to the Production of the Anticancer Agent, Indirubin
|
|---|---|
| Published in |
Molecular Biotechnology, March 2015
|
| DOI | 10.1007/s12033-015-9863-4 |
| Pubmed ID | |
| Authors |
Lampet Wongsaroj, Ratiboot Sallabhan, James M. Dubbs, Skorn Mongkolsuk, Suvit Loprasert |
| Abstract |
Indirubin is a strong inhibitor of several eukaryotic cell signaling pathways and shows promise as a treatment for myelocytic leukemia and Alzheimer's disease. The tmoABCDEF operon, encoding the components of a novel toluene 4-monooxygenase from the paint factory soil isolate, Pseudomonas sp. M4, was cloned and expressed in Escherichia coli. E. coli::pKSR12 expressing the tmo genes was used to develop a two-phase [dioctyl phthalate (DOP)/aqueous medium] culture system that was optimized to obtain maximal yields of indirubin from the starting substrate, indole. DOP was used as the organic phase to solubilize and sequester the toxic indole substrate, making possible the use of high indole concentrations that would otherwise interfere with growth in aqueous media. A 50 % (v/v) DOP two-phase system using tryptophan medium containing 3 mM cysteine, 5 mM indole, and 1 mM isatin yielded 102.4 mg/L of indirubin with no conversion of indole to the less valuable alternate product, indigo. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 3 | 13% |
| Student > Master | 3 | 13% |
| Student > Bachelor | 2 | 8% |
| Other | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 7 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 17% |
| Biochemistry, Genetics and Molecular Biology | 4 | 17% |
| Psychology | 3 | 13% |
| Chemical Engineering | 1 | 4% |
| Environmental Science | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 10 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2015.
All research outputs
#18,403,994
of 22,796,179 outputs
Outputs from Molecular Biotechnology
#735
of 959 outputs
Outputs of similar age
#209,139
of 286,004 outputs
Outputs of similar age from Molecular Biotechnology
#8
of 19 outputs
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