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The proteomes of transcription factories containing RNA polymerases I, II or III

Overview of attention for article published in Nature Methods, September 2011
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
7 X users
wikipedia
3 Wikipedia pages

Citations

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74 Dimensions

Readers on

mendeley
166 Mendeley
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1 CiteULike
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Title
The proteomes of transcription factories containing RNA polymerases I, II or III
Published in
Nature Methods, September 2011
DOI 10.1038/nmeth.1705
Pubmed ID
Authors

Svitlana Melnik, Binwei Deng, Argyris Papantonis, Sabyasachi Baboo, Ian M Carr, Peter R Cook

Abstract

Human nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammalian cells. After isolation, we analyzed their protein content by mass spectrometry. Each complex represents part of the core of a transcription factory. For example, the RNA polymerase II complex contains subunits unique to RNA polymerase II plus various transcription factors but shares a number of ribonucleoproteins with the other polymerase complexes; it is also rich in polymerase II transcripts. We also describe a native chromosome conformation capture method to confirm that the complexes remain attached to the same pairs of DNA templates found in vivo.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 166 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 5 3%
United States 3 2%
Germany 1 <1%
France 1 <1%
Israel 1 <1%
Portugal 1 <1%
Russia 1 <1%
Canada 1 <1%
Unknown 152 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 60 36%
Student > Ph. D. Student 37 22%
Student > Master 14 8%
Professor > Associate Professor 13 8%
Student > Bachelor 10 6%
Other 17 10%
Unknown 15 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 85 51%
Biochemistry, Genetics and Molecular Biology 46 28%
Chemistry 6 4%
Physics and Astronomy 3 2%
Computer Science 2 1%
Other 6 4%
Unknown 18 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 March 2022.
All research outputs
#5,346,012
of 25,396,120 outputs
Outputs from Nature Methods
#3,400
of 5,354 outputs
Outputs of similar age
#28,882
of 142,204 outputs
Outputs of similar age from Nature Methods
#41
of 72 outputs
Altmetric has tracked 25,396,120 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,354 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.5. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,204 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.