| Title |
A Plant-Derived Multi-HIV Antigen Induces Broad Immune Responses in Orally Immunized Mice
|
|---|---|
| Published in |
Molecular Biotechnology, March 2015
|
| DOI | 10.1007/s12033-015-9856-3 |
| Pubmed ID | |
| Authors |
Néstor Rubio-Infante, Dania O. Govea-Alonso, Andrea Romero-Maldonado, Ana Lilia García-Hernández, Damaris Ilhuicatzi-Alvarado, Jorge A. Salazar-González, Schuyler S. Korban, Sergio Rosales-Mendoza, Leticia Moreno-Fierros |
| Abstract |
Multi-HIV, a multiepitopic protein derived from both gp120 and gp41 envelope proteins of the human immunodeficiency virus (HIV), has been proposed as a vaccine prototype capable of inducing broad immune responses, as it carries various B and T cell epitopes from several HIV strains. In this study, the immunogenic properties of a Multi-HIV expressed in tobacco chloroplasts are evaluated in test mice. BALB/c mice orally immunized with tobacco-derived Multi-HIV have elicited antibody responses, including both the V3 loop of gp120 and the ELDKWA epitope of gp41. Based on splenocyte proliferation assays, stimulation with epitopes of the C4, V3 domain of gp120, and the ELDKWA domain of gp41 elicits positive cellular responses. Furthermore, specific interferon gamma production is observed in both CD4+ and CD8+ T cells stimulated with HIV peptides. These results demonstrate that plant-derived Multi-HIV induces T helper-specific responses. Altogether, these findings illustrate the immunogenic potential of plant-derived Multi-HIV in an oral immunization scheme. The potential of this low-cost immunization approach and its implications on HIV/AIDS vaccine development are discussed. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 53 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 16 | 30% |
| Researcher | 8 | 15% |
| Student > Ph. D. Student | 5 | 9% |
| Student > Master | 4 | 8% |
| Student > Postgraduate | 3 | 6% |
| Other | 6 | 11% |
| Unknown | 11 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 26% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Social Sciences | 5 | 9% |
| Medicine and Dentistry | 5 | 9% |
| Immunology and Microbiology | 2 | 4% |
| Other | 7 | 13% |
| Unknown | 12 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2015.
All research outputs
#18,403,994
of 22,796,179 outputs
Outputs from Molecular Biotechnology
#735
of 959 outputs
Outputs of similar age
#209,339
of 286,345 outputs
Outputs of similar age from Molecular Biotechnology
#8
of 19 outputs
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