| Title |
The first anionic defensin from amphibians
|
|---|---|
| Published in |
Amino Acids, March 2015
|
| DOI | 10.1007/s00726-015-1963-8 |
| Pubmed ID | |
| Authors |
Lin Wei, Helong Che, Yi Han, Jing Lv, Lixian Mu, Lechun Lv, Jing Wu, Hailong Yang |
| Abstract |
A variety of antimicrobial peptides against infection have been identified from the skin of amphibians. However, knowledge on amphibian defensins is very limited. A novel anionic defensin designated PopuDef was purified from the skin of tree frog Polypedates puerensis, and the cDNA encoding PopuDef precursor was cloned from the skin cDNA library. The amino acid sequence of PopuDef (net charge: -2, pI: 4.75) shared the highest identity of 57 % (25/44) with the salamander defensin CFBD-1 (net charge: 0, pI: 6.14) from urodela amphibians. PopuDef showed moderate antimicrobial activities against P. aeruginosa and S. aureus (MICs are 19.41 and 17.25 μM, respectively), and relatively weak activities against E. coli and B. subtilis (MICs are 38.82 and 43.14 μM, respectively). Tissue distribution analysis indicated that relatively high expression level of PopuDef mRNA was observed in immune-related tissues including skin, gut, lung and spleen. Furthermore, the expression level of PopuDef was significantly upregulated in these tissues after tree frogs were infected with different bacteria strains mentioned above. Interestingly, the induction of PopuDef challenged with E. coli or B. subtilis, which was less sensitive to PopuDef, was much higher than that did with P. aeruginosa or S. aureus. These findings highlight the key role of PopuDef in innate immunity against infection. To our knowledge, PopuDef is the first anionic defensin characterized from amphibians. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Belgium | 1 | 3% |
| Unknown | 34 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 19% |
| Student > Master | 7 | 19% |
| Researcher | 5 | 14% |
| Student > Doctoral Student | 3 | 8% |
| Student > Postgraduate | 2 | 6% |
| Other | 4 | 11% |
| Unknown | 8 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 36% |
| Agricultural and Biological Sciences | 7 | 19% |
| Environmental Science | 2 | 6% |
| Immunology and Microbiology | 2 | 6% |
| Mathematics | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 10 | 28% |
Attention Score in Context
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#17,751,741
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Outputs from Amino Acids
#1,113
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#179,745
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Outputs of similar age from Amino Acids
#19
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