| Title |
Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
|
|---|---|
| Published in |
Chinese Journal of Integrative Medicine, April 2018
|
| DOI | 10.1007/s11655-018-2835-3 |
| Pubmed ID | |
| Authors |
Bin Zhang, Nan Chu, Xue-min Qiu, Wei Tang, Hans-Jürgen Gober, Da-jin Li, Ling Wang |
| Abstract |
To evaluate the effect of Heyan Kuntai Capsule (, HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group), including the control group (saline), 17 β-estradiol group [E2, 100 μg/(kg·d)], and low-, medium-, and high-dose of HYKT groups [0.3, 0.9, 2.7 g/(kg·d), respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits, REC8 meiotic recombination protein (REC8), structural maintenance of chromosome (SMC) 1 β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γ H2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles (P<0.05). Furthermore, it showed an increased trend in the levels of REC8 and SMC1 β, after administration with E2 and HYKT, and no obvious change in the level of SMC3. HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1 β) in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 40% |
| Professor | 1 | 20% |
| Student > Master | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 40% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Engineering | 1 | 20% |
| Unknown | 1 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 April 2018.
All research outputs
#15,505,836
of 23,043,346 outputs
Outputs from Chinese Journal of Integrative Medicine
#305
of 681 outputs
Outputs of similar age
#208,495
of 327,033 outputs
Outputs of similar age from Chinese Journal of Integrative Medicine
#6
of 13 outputs
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