| Title |
Where is TRPV1 expressed in the bladder, do we see the real channel?
|
|---|---|
| Published in |
Naunyn-Schmiedeberg's Archives of Pharmacology, January 2009
|
| DOI | 10.1007/s00210-008-0391-7 |
| Pubmed ID | |
| Authors |
Wouter Everaerts, M. Rosario Sepúlveda, Thomas Gevaert, Tania Roskams, Bernd Nilius, Dirk De Ridder |
| Abstract |
Transient receptor potential channel-vanilloid subfamily member 1 (TRPV1) is an important target in the treatment of bladder overactivity. This receptor is suggested to function as a mechanosensor in the normal bladder and to mediate the development of bladder overactivity during cystitis. Our aim was to determine the cellular distribution of TRPV1 in mouse and rat bladder tissue. We used three different commercial TRPV1 antibodies to perform immunohistochemistry on bladder tissue from rats and wild-type and TRPV1(-/-) mice, using trigeminal ganglia as a control tissue for TRPV1 expression. Although two of the antibodies seemed to react specifically in trigeminal ganglion tissue, all the antibodies produced a similar staining pattern in the urothelium of wild-type and TRPV1(-/-) mice. These data show that TRPV1 antibodies can cause an aspecific immunostaining in bladder tissue, urging for additional research to confirm the exact distribution of TRPV1 in bladder. In conclusion, we think that the use of negative controls on knockout mice, whenever available, is mandatory when conducting immunohistochemical localization studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 2 | 6% |
| Unknown | 34 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 31% |
| Student > Ph. D. Student | 7 | 19% |
| Professor > Associate Professor | 4 | 11% |
| Other | 3 | 8% |
| Lecturer | 2 | 6% |
| Other | 5 | 14% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 13 | 36% |
| Agricultural and Biological Sciences | 9 | 25% |
| Neuroscience | 3 | 8% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Business, Management and Accounting | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 5 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2015.
All research outputs
#5,881,941
of 22,796,179 outputs
Outputs from Naunyn-Schmiedeberg's Archives of Pharmacology
#306
of 1,724 outputs
Outputs of similar age
#39,529
of 170,416 outputs
Outputs of similar age from Naunyn-Schmiedeberg's Archives of Pharmacology
#4
of 7 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,724 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,416 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.