| Chapter title |
Flow Sorting Plant Chromosomes
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Cytogenetics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3622-9_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3620-5, 978-1-4939-3622-9
|
| Authors |
Jan Vrána, Petr Cápal, Jarmila Číhalíková, Marie Kubaláková, Jaroslav Doležel, Vrána, Jan, Cápal, Petr, Číhalíková, Jarmila, Kubaláková, Marie, Doležel, Jaroslav |
| Abstract |
Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting. |
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Mendeley readers
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