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Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins

Overview of attention for article published in Scientific Reports, March 2015
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  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

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Title
Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins
Published in
Scientific Reports, March 2015
DOI 10.1038/srep09583
Pubmed ID
Authors

Errin Johnson, Elena Seiradake, E. Yvonne Jones, Ilan Davis, Kay Grünewald, Rainer Kaufmann

Abstract

We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge. We identified key aspects of the sample preparation procedure of high pressure freezing, freeze substitution and resin embedding that are critical for preserving fluorescence and photo-switching of standard fluorescent proteins, such as mGFP, mVenus and mRuby2. This enabled us to combine single molecule localization microscopy with transmission electron microscopy imaging of standard fluorescent proteins in cryo-fixed resin embedded cells. We achieved a structural resolution of 40-50 nm (~17 nm average single molecule localization accuracy) in the fluorescence images without the use of chemical fixation or special fluorophores. Using this approach enabled the correlation of fluorescently labeled structures to the ultrastructure in the same cell at the nanometer level and superior structural preservation.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 231 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 2 <1%
Czechia 2 <1%
Germany 1 <1%
Netherlands 1 <1%
France 1 <1%
Sweden 1 <1%
Switzerland 1 <1%
Finland 1 <1%
United Kingdom 1 <1%
Other 2 <1%
Unknown 218 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 62 27%
Researcher 52 23%
Student > Master 18 8%
Student > Bachelor 16 7%
Professor 10 4%
Other 35 15%
Unknown 38 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 71 31%
Biochemistry, Genetics and Molecular Biology 52 23%
Engineering 15 6%
Physics and Astronomy 9 4%
Chemistry 9 4%
Other 37 16%
Unknown 38 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 October 2020.
All research outputs
#6,204,267
of 22,797,621 outputs
Outputs from Scientific Reports
#42,064
of 123,068 outputs
Outputs of similar age
#73,333
of 264,714 outputs
Outputs of similar age from Scientific Reports
#360
of 1,163 outputs
Altmetric has tracked 22,797,621 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 123,068 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,714 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 1,163 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.