Title |
Lengthening of the Stargazin Cytoplasmic Tail Increases Synaptic Transmission by Promoting Interaction to Deeper Domains of PSD-95
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Published in |
Neuron, April 2015
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DOI | 10.1016/j.neuron.2015.03.013 |
Pubmed ID | |
Authors |
Anne-Sophie Hafner, Andrew C. Penn, Dolors Grillo-Bosch, Natacha Retailleau, Christel Poujol, Amandine Philippat, Françoise Coussen, Matthieu Sainlos, Patricio Opazo, Daniel Choquet |
Abstract |
PSD-95 is a prominent organizer of the postsynaptic density (PSD) that can present a filamentous orientation perpendicular to the plasma membrane. Interactions between PSD-95 and transmembrane proteins might be particularly sensitive to this orientation, as "long" cytoplasmic tails might be required to reach deeper PSD-95 domains. Extension/retraction of transmembrane protein C-tails offer a new way of regulating binding to PSD-95. Using stargazin as a model, we found that enhancing the apparent length of stargazin C-tail through phosphorylation or by an artificial linker was sufficient to potentiate binding to PSD-95, AMPAR anchoring, and synaptic transmission. A linear extension of stargazin C-tail facilitates binding to PSD-95 by preferentially engaging interaction with the farthest located PDZ domains regarding to the plasma membrane, which present a greater affinity for the stargazin PDZ-domain-binding motif. Our study reveals that the concerted orientation of the stargazin C-tail and PSD-95 is a major determinant of synaptic strength. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
France | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Czechia | 2 | 1% |
Chile | 1 | <1% |
France | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 176 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 41 | 22% |
Student > Ph. D. Student | 37 | 20% |
Student > Bachelor | 21 | 11% |
Student > Master | 15 | 8% |
Professor | 10 | 5% |
Other | 22 | 12% |
Unknown | 37 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 56 | 31% |
Agricultural and Biological Sciences | 46 | 25% |
Biochemistry, Genetics and Molecular Biology | 29 | 16% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
Engineering | 3 | 2% |
Other | 9 | 5% |
Unknown | 37 | 20% |