| Title |
Time-sequential modulation in expression of growth factors from platelet-rich plasma (PRP) on the chondrocyte cultures
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, September 2011
|
| DOI | 10.1007/s11010-011-1081-1 |
| Pubmed ID | |
| Authors |
Se-Il Park, Hye-Rim Lee, Sukyoung Kim, Myun-Whan Ahn, Sun Hee Do |
| Abstract |
Platelets are involved in hemostasis, wound healing, and tumor growth. Autologous blood products are commonly used to facilitate healing in a variety of clinical surgery applications. Recently, it was shown that platelet-rich plasma (PRP) has more specific growth factors that participate in the healing process. This study investigated the expression of PRP growth factors and evaluated their potential role in the cartilage regeneration using primary isolated chondrocytes. PRP obtained from New Zealand White rabbit by low speed centrifugation. Extracted PRPs contained 6-10 × 10(6) platelet/μl and concentration of platelets was slightly variable. Primary isolated chondrocytes from the same rabbits were cultured and treated with 0.1-20% PRP. The cells were collected and examined by reverse transcription-polymerase chain reaction and cytochemical staining. The expression of sex determining region Y-box 9, transforming growth factor-beta, vascular endothelial growth factor, and chondromdulin-I was increased in chondrocyte cultures with 10% PRP by time-dependent manner. To maintain the integrity of the cartilage, the proteoglycan contents were also up-regulated from the mRNA of aggrecan and positive Safranin-O staining in PRP concentration- and time-dependent manner. PRP provides crucial growth factors related to chondrocyte proliferation and differentiation through time-sequential modulation. Controlled in vivo trials for cartilage regeneration are needed. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Mexico | 1 | 2% |
| United States | 1 | 2% |
| Unknown | 51 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 24% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Master | 5 | 9% |
| Other | 4 | 7% |
| Student > Postgraduate | 4 | 7% |
| Other | 8 | 15% |
| Unknown | 12 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 21 | 39% |
| Agricultural and Biological Sciences | 6 | 11% |
| Biochemistry, Genetics and Molecular Biology | 4 | 7% |
| Neuroscience | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 14 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2011.
All research outputs
#14,719,073
of 22,653,392 outputs
Outputs from Molecular and Cellular Biochemistry
#1,249
of 2,287 outputs
Outputs of similar age
#89,287
of 131,769 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#19
of 31 outputs
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