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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Novel DNA Biosensor Using a Ferrocenyl Intercalator Applied to the Potential Detection of Human Population Biomarkers in Wastewater
|
|---|---|
| Published in |
Environmental Science & Technology, April 2015
|
| DOI | 10.1021/acs.est.5b00637 |
| Pubmed ID | |
| Authors |
Zhugen Yang, Marc Anglès d’Auriac, Sean Goggins, Barbara Kasprzyk-Hordern, Kevin V. Thomas, Christopher G. Frost, Pedro Estrela |
| Abstract |
A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA (dsDNA) intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol (MCH) to control the surface density of the ssDNA probe, and hybridized with complementary DNA. The binding of the Fc intercalator to dsDNA was measured by differential pulse voltammetry (DPV). This new biosensor was optimized to allow the detection of single base pair mismatched sequences and, able to detect as low as 10 pM target ssDNA with a dynamic range from 10 pM to 100 nM. DNA extracted from wastewater was analysed by quantitative polymerase chain reaction (qPCR) targeting human-specific mitochondrial DNA (mtDNA). The aim of this approach is to enable the analysis of population biomarkers in wastewater for the evaluation of public health using wastewater-based epidemiology (WBE). The E-DNA biosensor was employed to detect human-specific mtDNA from wastewater before and after PCR amplification. The results demonstrate the feasibility of detecting DNA biomarkers in wastewater using the developed biosensor, which may allow the further development of DNA population biomarkers for public health using WBE. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Spain | 1 | 1% |
| Unknown | 68 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 23% |
| Researcher | 13 | 18% |
| Student > Master | 9 | 13% |
| Professor | 4 | 6% |
| Other | 2 | 3% |
| Other | 8 | 11% |
| Unknown | 19 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 19 | 27% |
| Environmental Science | 6 | 8% |
| Agricultural and Biological Sciences | 5 | 7% |
| Engineering | 5 | 7% |
| Biochemistry, Genetics and Molecular Biology | 2 | 3% |
| Other | 7 | 10% |
| Unknown | 27 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2015.
All research outputs
#20,657,128
of 25,377,790 outputs
Outputs from Environmental Science & Technology
#18,394
of 20,675 outputs
Outputs of similar age
#207,506
of 279,911 outputs
Outputs of similar age from Environmental Science & Technology
#234
of 279 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,675 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.8. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,911 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 279 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.