Title |
Nasal carriage of Staphylococcus aureus in healthy humans with different levels of contact with animals in Tunisia: genetic lineages, methicillin resistance, and virulence factors
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Published in |
European Journal of Clinical Microbiology & Infectious Diseases, November 2010
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DOI | 10.1007/s10096-010-1109-6 |
Pubmed ID | |
Authors |
K. Ben Slama, H. Gharsa, N. Klibi, A. Jouini, C. Lozano, E. Gómez-Sanz, M. Zarazaga, A. Boudabous, C. Torres |
Abstract |
Nasal swabs of 423 healthy humans who showed different levels of contact with animals (frequent, 168; sporadic, 94; no contact, 161) were obtained in Tunisia (2008-2009), and 99 of them presented other associated risk factors. Methicillin-resistant Staphylococcus aureus (MRSA) was detected in one of these 423 samples (0.24%), retrieved from a veterinarian. The MRSA isolate was mecA-positive, typed as ST80-t203-SCCmecIVc-agrIII, and contained tet(K), ant(6)-Ia, and aph(3')-IIIa genes encoding tetracycline, streptomycin, and kanamycin resistance, respectively. This MRSA isolate also contained the lukF/lukS virulence gene encoding Panton-Valentine leukocidin. Fifty-four (12.8%) additional nasal samples contained methicillin-susceptible S. aureus (MSSA) and one isolate/sample was characterized. A high diversity of spa types (n = 43; 4 new) and pulsed-field gel electrophoresis (PFGE) types (n = 37) was detected among the 55 recovered S. aureus strains. The percentages of antimicrobial resistance/detected resistance genes were as follows: tetracycline [22%/tet(K)-tet(L)-tet(M)], erythromycin [5%/msrA], ciprofloxacin [14.5%], trimethoprim-sulfamethoxazole [2%/dfrA], streptomycin [11%/ant(6)-Ia], kanamycin [7%/aph(3')-IIIa], amikacin [5%], and chloramphenicol [2%]. Four and two isolates carried the lukF/lukS and eta and/or etb genes, respectively, and always in individuals with contact with animals. Eleven isolates carried the tst gene and were recovered from individuals with different levels of contact with animals. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 1% |
India | 1 | 1% |
Denmark | 1 | 1% |
Belgium | 1 | 1% |
Unknown | 65 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 19 | 28% |
Student > Master | 9 | 13% |
Student > Doctoral Student | 8 | 12% |
Professor | 6 | 9% |
Student > Ph. D. Student | 6 | 9% |
Other | 10 | 14% |
Unknown | 11 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 23% |
Medicine and Dentistry | 14 | 20% |
Immunology and Microbiology | 13 | 19% |
Veterinary Science and Veterinary Medicine | 3 | 4% |
Biochemistry, Genetics and Molecular Biology | 3 | 4% |
Other | 2 | 3% |
Unknown | 18 | 26% |