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A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids

Overview of attention for article published in BMC Biophysics, April 2015
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Title
A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids
Published in
BMC Biophysics, April 2015
DOI 10.1186/s13628-015-0019-5
Pubmed ID
Authors

Phuong H Nguyen, Anna M Popova, Kálmán Hideg, Peter Z Qin

Abstract

Spin labels, which are chemically stable radicals attached at specific sites of a bio-molecule, enable investigations on structure and dynamics of proteins and nucleic acids using techniques such as site-directed spin labeling and paramagnetic NMR. Among spin labels developed, the class of rigid labels have limited or no independent motions between the radical bearing moiety and the target, and afford a number of advantages in measuring distances and monitoring local dynamics within the parent bio-molecule. However, a general method for attaching a rigid label to nucleic acids in a nucleotide-independent manner has not been reported. We developed an approach for installing a nearly rigid nitroxide spin label, designated as R5c, at a specific site of the nucleic acid backbone in a nucleotide-independent manner. The method uses a post-synthesis approach to covalently attach the nitroxide moiety in a cyclic fashion to phosphorothioate groups introduced at two consecutive nucleotides of the target strand. R5c-labeled nucleic acids are capable of pairing with their respective complementary strands, and the cyclic nature of R5c attachment significantly reduced independence motions of the label with respect to the parent duplex, although it may cause distortion of the local environment at the site of labeling. R5c yields enhanced sensitivity to the collective motions of the duplex, as demonstrated by its capability to reveal changes in collective motions of the substrate recognition duplex of the 120-kDa Tetrahymena group I ribozyme, which elude detection by a flexible label. The cyclic R5c nitroxide can be efficiently attached to a target nucleic acid site using a post-synthetic coupling approach conducted under mild biochemical conditions, and serves as a viable label for experimental investigation of segmental motions in nucleic acids, including large folded RNAs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 5%
Netherlands 1 5%
Unknown 20 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 41%
Researcher 3 14%
Student > Doctoral Student 2 9%
Lecturer > Senior Lecturer 1 5%
Other 1 5%
Other 2 9%
Unknown 4 18%
Readers by discipline Count As %
Chemistry 12 55%
Biochemistry, Genetics and Molecular Biology 3 14%
Business, Management and Accounting 1 5%
Social Sciences 1 5%
Agricultural and Biological Sciences 1 5%
Other 0 0%
Unknown 4 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2015.
All research outputs
#14,102,908
of 23,881,329 outputs
Outputs from BMC Biophysics
#27
of 57 outputs
Outputs of similar age
#131,406
of 267,052 outputs
Outputs of similar age from BMC Biophysics
#3
of 5 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 57 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,052 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.