| Title |
MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway
|
|---|---|
| Published in |
Tumor Biology, April 2015
|
| DOI | 10.1007/s13277-015-3409-z |
| Pubmed ID | |
| Authors |
Liang Shen, Chunming Sun, Yanyan Li, Xuetao Li, Ting Sun, Chuanjin Liu, Youxin Zhou, Ziwei Du |
| Abstract |
Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and its resistance to chemotherapy or radiotherapy. The mammalian target of rapamycin (mTOR) is highly expressed and regulates cellular proliferation and cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription and translation via up-regulating or down-regulating the levels of miRNAs. This study was conducted to explore the molecular functions of miR-199a-3p in glioma. We detected the expression of miR-199a-3p in glioma samples by quantitative PCR (qPCR). Then, we transfected the U87 and U251 cell lines with miR-199a-3p. Cellular proliferation, invasion, and apoptosis were assessed to explain the function of miR-199a-3p. PCR confirmed that the expression of miR-199a-3p was lower in glioma samples combined with normal brain tissues. The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability. Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p. MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 17% |
| Researcher | 6 | 17% |
| Student > Bachelor | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Other | 2 | 6% |
| Other | 3 | 8% |
| Unknown | 10 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 25% |
| Medicine and Dentistry | 7 | 19% |
| Agricultural and Biological Sciences | 4 | 11% |
| Neuroscience | 1 | 3% |
| Materials Science | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 13 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2015.
All research outputs
#18,405,972
of 22,799,071 outputs
Outputs from Tumor Biology
#1,369
of 2,622 outputs
Outputs of similar age
#193,644
of 264,944 outputs
Outputs of similar age from Tumor Biology
#62
of 152 outputs
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