Title |
Engineering monocyte-derived dendritic cells to secrete interferon-α enhances their ability to promote adaptive and innate anti-tumor immune effector functions
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Published in |
Cancer Immunology, Immunotherapy, April 2015
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DOI | 10.1007/s00262-015-1688-2 |
Pubmed ID | |
Authors |
Yannick Willemen, Johan M. J. Van den Bergh, Eva Lion, Sébastien Anguille, Vicky A. E. Roelandts, Heleen H. Van Acker, Steven D. I. Heynderickx, Barbara M. H. Stein, Marc Peeters, Carl G. Figdor, Viggo F. I. Van Tendeloo, I. Jolanda de Vries, Gosse J. Adema, Zwi N. Berneman, Evelien L. J. Smits |
Abstract |
Dendritic cell (DC) vaccination has demonstrated potential in clinical trials as a new effective cancer treatment, but objective and durable clinical responses are confined to a minority of patients. Interferon (IFN)-α, a type-I IFN, can bolster anti-tumor immunity by restoring or increasing the function of DCs, T cells and natural killer (NK) cells. Moreover, type-I IFN signaling on DCs was found to be essential in mice for tumor rejection by the innate and adaptive immune system. Targeted delivery of IFN-α by DCs to immune cells could boost the generation of anti-tumor immunity, while avoiding the side effects frequently associated with systemic administration. Naturally circulating plasmacytoid DCs, major producers of type-I IFN, were already shown capable of inducing tumor antigen-specific T cell responses in cancer patients without severe toxicity, but their limited number complicates their use in cancer vaccination. In the present work, we hypothesized that engineering easily generated human monocyte-derived mature DCs to secrete IFN-α using mRNA electroporation enhances their ability to promote adaptive and innate anti-tumor immunity. Our results show that IFN-α mRNA electroporation of DCs significantly increases the stimulation of tumor antigen-specific cytotoxic T cell as well as anti-tumor NK cell effector functions in vitro through high levels of IFN-α secretion. Altogether, our findings mark IFN-α mRNA-electroporated DCs as potent inducers of both adaptive and innate anti-tumor immunity and pave the way for clinical trial evaluation in cancer patients. |
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Country | Count | As % |
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Canada | 1 | 13% |
United Kingdom | 1 | 13% |
Unknown | 6 | 75% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 2 | 25% |
Scientists | 1 | 13% |
Mendeley readers
Geographical breakdown
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Spain | 1 | 2% |
United States | 1 | 2% |
Unknown | 44 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 6 | 13% |
Student > Master | 6 | 13% |
Student > Ph. D. Student | 6 | 13% |
Researcher | 5 | 11% |
Student > Bachelor | 5 | 11% |
Other | 8 | 17% |
Unknown | 10 | 22% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 12 | 26% |
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Biochemistry, Genetics and Molecular Biology | 6 | 13% |
Business, Management and Accounting | 1 | 2% |
Other | 2 | 4% |
Unknown | 12 | 26% |