| Title |
Trans-splicing of plastid rps12 transcripts, mediated by AtPPR4, is essential for embryo patterning in Arabidopsis thaliana
|
|---|---|
| Published in |
Planta, April 2018
|
| DOI | 10.1007/s00425-018-2896-8 |
| Pubmed ID | |
| Authors |
Luca Tadini, Roberto Ferrari, Marie-Kristin Lehniger, Chiara Mizzotti, Fabio Moratti, Francesca Resentini, Monica Colombo, Alex Costa, Simona Masiero, Paolo Pesaresi |
| Abstract |
AtPPR4-mediated trans-splicing of plastid rps12 transcripts is essential for key embryo morphogenetic events such as development of cotyledons, determination of provascular tissue, and organization of the shoot apical meristem (SAM), but not for the formation of the protodermal layer. Members of the pentatricopeptide repeat (PPR) containing protein family have emerged as key regulators of the organelle post-transcriptional processing and to be essential for proper plant embryo development. In this study, we report the functional characterization of the AtPPR4 (At5g04810) gene encoding a plastid nucleoid PPR protein. In-situ hybridization analysis reveals the presence of AtPPR4 transcripts already at the transition stage of embryo development. As a consequence, embryos lacking the AtPPR4 protein arrest their development at the transition/early-heart stages and show defects in the determination of the provascular tissue and organization of SAM. This complex phenotype is due to the specific role of AtPPR4 in the trans-splicing of the plastid rps12 transcripts, as shown by northern and slot-blot hybridizations, and the consequent defect in 70S ribosome accumulation and plastid protein synthesis, in agreement with the role proposed for the maize orthologue, ZmPPR4. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 3 | 38% |
| Spain | 1 | 13% |
| United States | 1 | 13% |
| Unknown | 3 | 38% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 63% |
| Scientists | 3 | 38% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 35% |
| Researcher | 7 | 30% |
| Other | 1 | 4% |
| Professor | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 35% |
| Agricultural and Biological Sciences | 8 | 35% |
| Medicine and Dentistry | 2 | 9% |
| Social Sciences | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 September 2018.
All research outputs
#3,955,272
of 23,926,844 outputs
Outputs from Planta
#170
of 2,807 outputs
Outputs of similar age
#75,448
of 329,580 outputs
Outputs of similar age from Planta
#1
of 36 outputs
Altmetric has tracked 23,926,844 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,807 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 93% of its peers.
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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.