Title |
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
|
---|---|
Published in |
ACS Synthetic Biology, May 2015
|
DOI | 10.1021/sb500366v |
Pubmed ID | |
Authors |
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, John E. Dueber |
Abstract |
Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology due to its long history in industrial fermentations. However, until recently, most synthetic biology systems have focused on bacteria. While there is a wealth of resources and literature about the biology of yeast, it can be daunting to navigate and extract the tools needed for engineering applications. Here we present a versatile engineering platform for yeast, which contains both a rapid, modular assembly method and a basic set of characterized parts. This platform provides a framework in which to create new designs, as well as data on promoters, terminators, degradation tags, and copy number to inform those designs. Additionally, we describe genome-editing tools for making modifications directly to the yeast chromosomes, which we find preferable to plasmids due to reduced variability in expression. With this toolkit, we strive to simplify the process of engineering yeast by standardizing the physical manipulations and suggesting best practices that together will enable more straightforward translation of materials and data from one group to another. Additionally, by relieving researchers of the burden of technical details, they can focus on higher-level aspects of experimental design. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 13 | 34% |
Canada | 2 | 5% |
United Kingdom | 2 | 5% |
Finland | 1 | 3% |
Italy | 1 | 3% |
Australia | 1 | 3% |
Thailand | 1 | 3% |
Mexico | 1 | 3% |
Germany | 1 | 3% |
Other | 0 | 0% |
Unknown | 15 | 39% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 22 | 58% |
Scientists | 13 | 34% |
Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Unknown | 1 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 8 | <1% |
Canada | 3 | <1% |
France | 1 | <1% |
United Kingdom | 1 | <1% |
Indonesia | 1 | <1% |
Slovenia | 1 | <1% |
Switzerland | 1 | <1% |
China | 1 | <1% |
Belgium | 1 | <1% |
Other | 0 | 0% |
Unknown | 1466 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 294 | 20% |
Student > Bachelor | 219 | 15% |
Researcher | 213 | 14% |
Student > Master | 211 | 14% |
Student > Doctoral Student | 67 | 5% |
Other | 152 | 10% |
Unknown | 328 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 581 | 39% |
Agricultural and Biological Sciences | 315 | 21% |
Engineering | 68 | 5% |
Chemistry | 35 | 2% |
Chemical Engineering | 30 | 2% |
Other | 90 | 6% |
Unknown | 365 | 25% |