| Title |
LDH-C can be differentially expressed during fermentation of CHO cells
|
|---|---|
| Published in |
BMC Proceedings, November 2011
|
| DOI | 10.1186/1753-6561-5-s8-p107 |
| Pubmed ID | |
| Authors |
Berthold Szperalski, Christine Jung, Zhixin Shao, Anne Kantardjieff, Wei-Shou Hu |
| Abstract |
Expression of CHO mRNA was measured with special microarrays from the Consortium for Chinese Hamster Ovary (CHO) Cell Genomics led by Prof. Wei-Shou Hu of the University of Minnesota and Prof. Miranda Yap of the Bioprocess Technology Institute of A*STAR, Singapore (http://hugroup.cems.umn.edu/CHO/cho_index.html). Cultivation experiments were performed in small scale 2L stirred tank bioreactors. During fermentation a temperature shift of -3°C was performed. This was accompanied by a reduction of the cell specific lactate production rate. The analysis of transcriptome samples before and after the temperature shift with microarrays showed several changes in the expression of available gene markers. LDH-C expression raised about 2 fold after temperature shift. LDH-A did not change. As LDH-C is known to be a specialized isoenzyme in sperm cells for consuming lactate in a lactate containing milieu, LDH-C could be proposed as a target for genetic engineering, facilitating lactate consumption in the late phase of high cell density cultures and prolonging longevity of CHO production cultures by reducing lactate and base accumulation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 33% |
| Researcher | 6 | 29% |
| Other | 3 | 14% |
| Student > Master | 2 | 10% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 33% |
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Chemical Engineering | 3 | 14% |
| Engineering | 2 | 10% |
| Physics and Astronomy | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |
Attention Score in Context
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#20,269,439
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Outputs from BMC Proceedings
#319
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Outputs of similar age from BMC Proceedings
#32
of 44 outputs
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